Cattle in vitro induced pluripotent stem cells generated and maintained in 5 or 20% oxygen and different supplementation

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dc.contributor.authorBessi, Brendon Willian
dc.contributor.authorBotigelli, Ramon Cesar [UNESP]
dc.contributor.authorPieri, Naira Caroline Godoy
dc.contributor.authorMachado, Lucas Simões
dc.contributor.authorCruz, Jessica Brunhara
dc.contributor.authorde Moraes, Pamela
dc.contributor.authorde Souza, Aline Fernanda
dc.contributor.authorRecchia, Kaiana
dc.contributor.authorBarbosa, Gabriela
dc.contributor.authorde Castro, Raquel Vasconcelos Guimarães [UNESP]
dc.contributor.authorNogueira, Marcelo Fábio Gouveia [UNESP]
dc.contributor.authorBressan, Fabiana Fernandes
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:30:43Z
dc.date.available2022-04-29T08:30:43Z
dc.date.issued2021-06-01
dc.description.abstractThe event of cellular reprogramming into pluripotency is influenced by several factors, such as in vitro culture conditions (e.g., culture medium and oxygen concentration). Herein, bovine iPSCs (biPSCs) were generated in different levels of oxygen tension (5% or 20% of oxygen) and supple-mentation (bFGF or bFGF + LIF + 2i—bFL2i) to evaluate the efficiency of pluripotency induction and maintenance in vitro. Initial reprogramming was observed in all groups and bFL2i supplementation initially resulted in a superior number of colonies. However, bFL2i supplementation in low oxygen led to a loss of self-renewal and pluripotency maintenance. All clonal lines were positive for alkaline phosphatase; they expressed endogenous pluripotency-related genes SOX2, OCT4 and STELLA. However, expression was decreased throughout the passages without the influence of oxygen tension. GLUT1 and GLUT3 were upregulated by low oxygen. The biPSCs were immunofluorescence-positive stained for OCT4 and SOX2 and they formed embryoid bodies which differentiated in ectoderm and mesoderm (all groups), as well as endoderm (one line from bFL2i in high oxygen). Our study is the first to compare high and low oxygen environments during and after induced reprogramming in cattle. In our conditions, a low oxygen environment did not favor the pluripotency maintenance of biPSCs.en
dc.description.affiliationDepartment of Veterinary Medicine Faculty of Animal Science and Food Engineering University of São Paulo (USP)
dc.description.affiliationDepartment of Pharmacology Institute of Biosciences (IBB) São Paulo State University (UNESP)
dc.description.affiliationDepartment of Animal Reproduction Faculty of Veterinary Medicine and Animal Sciences University of São Paulo (USP)
dc.description.affiliationDepartment of Pathology Reproduction and One Health Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
dc.description.affiliationDepartment of Biological Science School of Sciences Humanities and Languages São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Pharmacology Institute of Biosciences (IBB) São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Pathology Reproduction and One Health Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Biological Science School of Sciences Humanities and Languages São Paulo State University (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdFAPESP: 2012/50533-2
dc.description.sponsorshipIdFAPESP: 2015/26818-5
dc.description.sponsorshipIdFAPESP: 2016/16841-2
dc.description.sponsorshipIdFAPESP: 2018/24520-7
dc.description.sponsorshipIdFAPESP: 2019/10751-0
dc.description.sponsorshipIdCAPES: 23038.006964/2014-43
dc.description.sponsorshipIdCNPq: 433133/2018-0
dc.identifierhttp://dx.doi.org/10.3390/cells10061531
dc.identifier.citationCells, v. 10, n. 6, 2021.
dc.identifier.doi10.3390/cells10061531
dc.identifier.issn2073-4409
dc.identifier.scopus2-s2.0-85110284434
dc.identifier.urihttp://hdl.handle.net/11449/229144
dc.language.isoeng
dc.relation.ispartofCells
dc.sourceScopus
dc.subjectAcquisition of pluripotency
dc.subjectBovine iPSCs
dc.subjectOxygen
dc.subjectPluripotent state
dc.titleCattle in vitro induced pluripotent stem cells generated and maintained in 5 or 20% oxygen and different supplementationen
dc.typeArtigo

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