Influence of supplement administration of omega-3 on the subcutaneous tissue response of endodontic sealers in Wistar rats

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dc.contributor.authorde Oliveira, Pedro Henrique Chaves [UNESP]
dc.contributor.authorGomes Filho, João Eduardo [UNESP]
dc.contributor.authorRodrigues, Michael Júnio da Silva [UNESP]
dc.contributor.authorda Silva, Cristiane Cantiga [UNESP]
dc.contributor.authorCardoso, Carolina da Barros Moraes [UNESP]
dc.contributor.authorCosme daSilva, Leopoldo [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorCintra, Luciano Tavares Angelo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionAlagoas Federal University (UFAL)
dc.date.accessioned2023-03-01T20:57:12Z
dc.date.available2023-03-01T20:57:12Z
dc.date.issued2022-10-01
dc.description.abstractAim: Natural substances such as omega-3 have been used in the medical field due to their numerous properties and, in particular, modulating effect on the systemic and local inflammatory processes. Thus, this study evaluated the influence of omega-3 supplementation on the subcutaneous tissue response of endodontic sealers in Wistar Rats. Methodology: Polyethylene tubes were implanted in the subcutaneous tissue of 48 animals (one empty for control and three filled with Sealapex, AH Plus or Endofill). The animals were treated with omega-3 (TO) or water (TW). Treatments started 15 days before implantation until euthanasia. After 5, 15 and 30 days (n = 8), animals were euthanized and polyethylene tubes and surrounding tissue were removed and processed for histological analysis. The inflammatory reaction was analysed by Haematoxylin and Eosin stain and immunolabelling for IL-6 and TNF-α. The collagen maturity was analysed by picrosirius red stain and calcium deposition by von Kossa stain and polarized light. Results were statistically analysed (p <.05). Results: Amongst TW sealer groups, Endofill evoked a more intense inflammatory infiltrate compared with AH Plus and control in the 30-day period (p =.009). However, in TO sealer groups, there was no difference amongst the sealers and control in all periods (p >.05). Comparing each sealer as a function of the supplementation with water or omega-3, there are differences for Endofill (p =.001) and Sealapex (p =.005) in the 30-day period, presenting lower inflammatory infiltrate in the animals treated with omega-3. A higher percentage of immature fibres was observed at 15 and 30 days in the TO group, compared with the TW group (p <.05). The deposition of calcium particles was observed only by Sealapex in all periods, despite the supplementation procedure. Conclusions: Omega-3 supplementation influence the tissue reactions of endodontic sealers, modulating inflammation, the immunolabelling of IL-6 and TNF-α, the repair process and it does not interfere with calcium deposition.en
dc.description.affiliationEndodontic Section Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationDepartment of Endodontics School of Dentistry Alagoas Federal University (UFAL)
dc.description.affiliationDepartment of Basic Science School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationDental Assistance Center for Disabled Persons (CAOE) of the São Paulo State University (UNESP) School of Dentistry
dc.description.affiliationUnespEndodontic Section Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationUnespDepartment of Basic Science School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationUnespDental Assistance Center for Disabled Persons (CAOE) of the São Paulo State University (UNESP) School of Dentistry
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdFAPESP: 2017/24713
dc.format.extent1026-1041
dc.identifierhttp://dx.doi.org/10.1111/iej.13795
dc.identifier.citationInternational Endodontic Journal, v. 55, n. 10, p. 1026-1041, 2022.
dc.identifier.doi10.1111/iej.13795
dc.identifier.issn1365-2591
dc.identifier.issn0143-2885
dc.identifier.scopus2-s2.0-85134070374
dc.identifier.urihttp://hdl.handle.net/11449/241331
dc.language.isoeng
dc.relation.ispartofInternational Endodontic Journal
dc.sourceScopus
dc.subjectendodontics
dc.subjectexperimental animal models
dc.subjectmaterial testing
dc.subjectroot canal filling material
dc.subjectWistar rats
dc.titleInfluence of supplement administration of omega-3 on the subcutaneous tissue response of endodontic sealers in Wistar ratsen
dc.typeArtigo
unesp.author.orcid0000-0002-1593-7926[1]
unesp.author.orcid0000-0001-5994-2287[2]
unesp.author.orcid0000-0003-4313-7149[4]
unesp.author.orcid0000-0002-4943-7022[5]
unesp.author.orcid0000-0002-5755-1933[6]
unesp.author.orcid0000-0003-4859-0583[7]
unesp.author.orcid0000-0003-2348-7846[8]

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