Dengue virus surveillance: Detection of DENV-4 in the city of Sao Jose do Rio Preto, SP, Brazil

dc.contributor.authorColombo, Tatiana Elias
dc.contributor.authorVedovello, Danila
dc.contributor.authorPacca-Mazaro, Carolina Colombelli
dc.contributor.authorMondini, Adriano
dc.contributor.authorAraujo, Joao Pessoa [UNESP]
dc.contributor.authorCabrera, Eliana
dc.contributor.authorLopes, Jose Carlos
dc.contributor.authorPenha dos Santos, Izalco Nuremberg
dc.contributor.authorNegri Reis, Andreia Francesli
dc.contributor.authorCosta, Fabiana Rodrigues
dc.contributor.authorArao Antonio Cruz, Lilian Elisa
dc.contributor.authorFerreira, Jurandir
dc.contributor.authorOliveira Rocha, Eliseu Soares de
dc.contributor.authorKroon, Erna Geessien
dc.contributor.authorMorais Bronzoni, Roberta Vieira de
dc.contributor.authorVasilakis, Nikos
dc.contributor.authorNogueira, Mauricio Lacerda
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionDept Vigilancia Saude
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionUniv Texas Med Branch
dc.description.abstractDengue viruses are the most common arbovirus infection worldwide and are caused by four distinct serotypes of the dengue virus (DENV). In the present study, we assessed DENV transmission in Sao Jose do Rio Preto (SJRP) from 2010 to 2014. We analyzed blood samples from febrile patients who were attended at health care centers in SJRP. DENV detection was performed using multiplex RT-PCR, using flavivirus generic primers, based on the genes of the non-structural protein (NS5), followed by nestedPCR assay with species-specific primers. We analyzed 1549 samples, of which 1389 were positive for NS1 by rapid test. One thousand and eight-seven samples (78%) were confirmed as positive by multiplex RTPCR: DENV-4, 48.5% (528/1087); DENV-1, 41.5% (449/1087); DENV-2, 9.5% (104/1087); and co-infection (5 DENV-1/DENV-4, 1 DENV-1/DENV-2), 0.5% (6/1087). Phylogenetic analysis of the DENV-4 grouped the isolates identified in this study with the American genotype and the showed a relationship between isolates from SJRP and isolates from the northern region of South America. Taken together, our data shows the detection and emergence of new dengue genotype in a new region and reiterate the importance of surveillance programs to detect and trace the evolution of DENV. (C) 2016 Elsevier B.V. All rights reserved.en
dc.description.affiliationFac Med Sao Jose do Rio Preto FAMERP, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Botucatu, Lab Virol, Botucatu, SP, Brazil
dc.description.affiliationDept Vigilancia Saude, Prefeitura Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Dept Microbiol, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Mato Grosso, Inst Ciencias Saude, Sinop, MT, Brazil
dc.description.affiliationUniv Texas Med Branch, Ctr Biodefense & Emerging Infect Dis, Ctr Trop Dis, Inst Human Infect & Immun, Galveston, TX 77555 USA
dc.description.affiliationUniv Texas Med Branch, Dept Pathol, Galveston, TX 77555 USA
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Botucatu, Lab Virol, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.identifier.citationActa Tropica. Amsterdam: Elsevier Science Bv, v. 164, p. 84-89, 2016.
dc.publisherElsevier B.V.
dc.relation.ispartofActa Tropica
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectDengue virus
dc.subjectPhylogenetic analysis
dc.subjectSurveillance programs
dc.titleDengue virus surveillance: Detection of DENV-4 in the city of Sao Jose do Rio Preto, SP, Brazilen
dcterms.rightsHolderElsevier B.V.[4][2][4]


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