Congenital toxoplasmosis in chronically infected and subsequently challenged ewes

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dc.contributor.authorDos Santos, Thaís Rabelo
dc.contributor.authorFaria, Gabriela Da Silva Magalhães [UNESP]
dc.contributor.authorGuerreiro, Bruna Martins [UNESP]
dc.contributor.authorDal Pietro, Nathalia Helena Pereira Da Silva [UNESP]
dc.contributor.authorLopes, Welber Daniel Zanetti [UNESP]
dc.contributor.authorDa Silva, Helenara Machado [UNESP]
dc.contributor.authorGarcia, João Luis
dc.contributor.authorLuvizotto, Maria Cecília Rui [UNESP]
dc.contributor.authorBresciani, Katia Denise Saraiva [UNESP]
dc.contributor.authorDa Costa, Alvimar José [UNESP]
dc.contributor.institutionRolim de Moura
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUEL
dc.date.accessioned2018-12-11T17:07:21Z
dc.date.available2018-12-11T17:07:21Z
dc.date.issued2016-10-01
dc.description.abstractThis experiment studied congenital transmission in sheep experimentally infected with oocysts of Toxoplasma gondii and reinfected at one of three stages of pregnancy. Twenty ewes were experimentally infected with T. gondii strain ME49 (day 0). After the T. gondii infection became chronic (IFAT≤512), the ewes were allocated with rams for coverage. After the diagnosis of pregnancy, these ewes were allocated into four experimental groups (n = 5): I-reinfected with T. gondii on the 40th day of gestation (DG); II-reinfected on DG 80; III-reinfected on DG 120; and IV-saline solution on DG 120 (not reinfected). Five ewes (IFAT<64) were kept as negative controls (uninfected, group V), therefore in groups I-III were infected prior to pregnancy and re-infected during pregnancy, group IV was only infected prior to pregnancy, and group V was not infected. Parasitism by T. gondii was investigated (histopathology, immunohistochemistry, mouse bioassay and PCR) in mothers and lambs tissue. All ewes produced lambs serologically positive for T. gondii. The results of the mouse bioassay, immunohistochemistry and PCR assays revealed the presence of T. gondii in all 20 sheep and their lambs. The congenital transmission of T. gondii was associated with fetal loss and abnormalities in persistently infected sheep and in ewes infected and subsequently reinfected by this protozoan. Therefore, congenital T. gondii infection was common when ewes were chronically infected prior to pregnancy, with or without reinfection during at various stages of gestation.en
dc.description.affiliationDepartment of Veterinary Medicine Rondônia Federal University (UNIR) Rolim de Moura, Av. Norte-Sul, 7300
dc.description.affiliationDepartment of Animal Pathology CPPAR FCAV UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/n
dc.description.affiliationDepartment of Preventive Veterinary Medicine UEL
dc.description.affiliationDepartment of Clinic Surgery and Animal Reproduction FMVA UNESP
dc.description.affiliationDepartment of Production and Animal Health FMVA UNESP
dc.description.affiliationUnespDepartment of Animal Pathology CPPAR FCAV UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/n
dc.description.affiliationUnespDepartment of Clinic Surgery and Animal Reproduction FMVA UNESP
dc.description.affiliationUnespDepartment of Production and Animal Health FMVA UNESP
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0165124
dc.identifier.citationPLoS ONE, v. 11, n. 10, 2016.
dc.identifier.doi10.1371/journal.pone.0165124
dc.identifier.file2-s2.0-84992709231.pdf
dc.identifier.issn1932-6203
dc.identifier.lattes5950594366829647
dc.identifier.lattes4584674909952477
dc.identifier.scopus2-s2.0-84992709231
dc.identifier.urihttp://hdl.handle.net/11449/173699
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleCongenital toxoplasmosis in chronically infected and subsequently challenged ewesen
dc.typeArtigo
unesp.author.lattes5950594366829647
unesp.author.lattes45872414870719291[10]
unesp.author.lattes4584674909952477

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Assis - CPPA - Centro de Pesquisa e Psicologia Aplicada
Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA