Integrative Analysis of the Ethanol Tolerance of Saccharomyces cerevisiae

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dc.contributor.authorWolf, Ivan Rodrigo [UNESP]
dc.contributor.authorMarques, Lucas Farinazzo [UNESP]
dc.contributor.authorde Almeida, Lauana Fogaça [UNESP]
dc.contributor.authorLázari, Lucas Cardoso [UNESP]
dc.contributor.authorde Moraes, Leonardo Nazário [UNESP]
dc.contributor.authorCardoso, Luiz Henrique [UNESP]
dc.contributor.authorAlves, Camila Cristina de Oliveira [UNESP]
dc.contributor.authorNakajima, Rafael Takahiro [UNESP]
dc.contributor.authorSchnepper, Amanda Piveta [UNESP]
dc.contributor.authorGolim, Marjorie de Assis [UNESP]
dc.contributor.authorCataldi, Thais Regiani
dc.contributor.authorNijland, Jeroen G.
dc.contributor.authorPinto, Camila Moreira [UNESP]
dc.contributor.authorFioretto, Matheus Naia [UNESP]
dc.contributor.authorAlmeida, Rodrigo Oliveira
dc.contributor.authorDriessen, Arnold J. M.
dc.contributor.authorSimōes, Rafael Plana [UNESP]
dc.contributor.authorLabate, Mônica Veneziano
dc.contributor.authorGrotto, Rejane Maria Tommasini [UNESP]
dc.contributor.authorLabate, Carlos Alberto
dc.contributor.authorFernandes Junior, Ary [UNESP]
dc.contributor.authorJustulin, Luis Antonio [UNESP]
dc.contributor.authorCoan, Rafael Luiz Buogo [UNESP]
dc.contributor.authorRamos, Érica [UNESP]
dc.contributor.authorFurtado, Fabiana Barcelos [UNESP]
dc.contributor.authorMartins, Cesar [UNESP]
dc.contributor.authorValente, Guilherme Targino
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Groningen
dc.contributor.institutionCiência e Tecnologia do Sudeste de Minas Gerais
dc.contributor.institutionMax Planck Institute for Heart and Lung Research
dc.date.accessioned2023-07-29T16:10:32Z
dc.date.available2023-07-29T16:10:32Z
dc.date.issued2023-03-01
dc.description.abstractEthanol (EtOH) alters many cellular processes in yeast. An integrated view of different EtOH-tolerant phenotypes and their long noncoding RNAs (lncRNAs) is not yet available. Here, large-scale data integration showed the core EtOH-responsive pathways, lncRNAs, and triggers of higher (HT) and lower (LT) EtOH-tolerant phenotypes. LncRNAs act in a strain-specific manner in the EtOH stress response. Network and omics analyses revealed that cells prepare for stress relief by favoring activation of life-essential systems. Therefore, longevity, peroxisomal, energy, lipid, and RNA/protein metabolisms are the core processes that drive EtOH tolerance. By integrating omics, network analysis, and several other experiments, we showed how the HT and LT phenotypes may arise: (1) the divergence occurs after cell signaling reaches the longevity and peroxisomal pathways, with CTA1 and ROS playing key roles; (2) signals reaching essential ribosomal and RNA pathways via SUI2 enhance the divergence; (3) specific lipid metabolism pathways also act on phenotype-specific profiles; (4) HTs take greater advantage of degradation and membraneless structures to cope with EtOH stress; and (5) our EtOH stress-buffering model suggests that diauxic shift drives EtOH buffering through an energy burst, mainly in HTs. Finally, critical genes, pathways, and the first models including lncRNAs to describe nuances of EtOH tolerance are reported here.en
dc.description.affiliationDepartment of Bioprocess and Biotechnology School of Agriculture São Paulo State University (UNESP)
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationLaboratory of Applied Biotechnology Clinical Hospital of the Medical School São Paulo State University (UNESP)
dc.description.affiliationDepartment of Parasitology Biomedical Sciences Institute University of São Paulo (USP)
dc.description.affiliationLaboratório Max Feffer de Genética de Plantas Escola Superior de Agricultura Luiz de Queiroz Universidade de São Paulo (USP)
dc.description.affiliationMolecular Microbiology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen, Nijenborgh 7
dc.description.affiliationZernike Institute for Advanced Materials University of Groningen, Nijenborgh 7
dc.description.affiliationInstituto Federal de Educação Ciência e Tecnologia do Sudeste de Minas Gerais, Campus Muriaé
dc.description.affiliationLaboratory of Bacteriology Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationDepartment of Biophysics and Pharmacology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationMax Planck Institute for Heart and Lung Research
dc.description.affiliationUnespDepartment of Bioprocess and Biotechnology School of Agriculture São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationUnespLaboratory of Applied Biotechnology Clinical Hospital of the Medical School São Paulo State University (UNESP)
dc.description.affiliationUnespLaboratory of Bacteriology Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Biophysics and Pharmacology Institute of Biosciences São Paulo State University (UNESP)
dc.description.sponsorshipUniversidade Estadual Paulista
dc.description.sponsorshipIdUniversidade Estadual Paulista: 12/2015-PROPe
dc.identifierhttp://dx.doi.org/10.3390/ijms24065646
dc.identifier.citationInternational Journal of Molecular Sciences, v. 24, n. 6, 2023.
dc.identifier.doi10.3390/ijms24065646
dc.identifier.issn1422-0067
dc.identifier.issn1661-6596
dc.identifier.scopus2-s2.0-85151949752
dc.identifier.urihttp://hdl.handle.net/11449/249837
dc.language.isoeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.sourceScopus
dc.subjectCRISPR–Cas9
dc.subjectdata integration
dc.subjectlncRNAs
dc.subjectlncRNA–protein interactions
dc.subjectmembraneless organelles
dc.subjectomics
dc.subjectsystems biology
dc.titleIntegrative Analysis of the Ethanol Tolerance of Saccharomyces cerevisiaeen
dc.typeArtigo
unesp.author.orcid0000-0002-9042-1823[1]
unesp.author.orcid0000-0001-7259-837X[2]
unesp.author.orcid0000-0003-4515-4137[5]
unesp.author.orcid0000-0003-2281-0867[6]
unesp.author.orcid0000-0002-9931-1102[8]
unesp.author.orcid0000-0002-5731-9085[9]
unesp.author.orcid0000-0002-4824-9441[10]
unesp.author.orcid0000-0002-9827-2017[11]
unesp.author.orcid0000-0002-7444-0864[15]
unesp.author.orcid0000-0001-9258-9104[16]
unesp.author.orcid0000-0002-3433-8574[17]
unesp.author.orcid0000-0002-4035-9486[19]
unesp.author.orcid0000-0002-2744-2824[21]
unesp.author.orcid0000-0001-6142-3515[22]
unesp.author.orcid0000-0003-4180-9415[23]
unesp.author.orcid0000-0003-1372-9370[24]
unesp.author.orcid0000-0003-3534-974X[26]
unesp.author.orcid0000-0001-5355-3424[27]

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