Serum protein concentrations in femaledogs submitted to ovariohysterectomy determined by means of SDS-page electro-phoresis

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dc.contributor.authorSchmidt, Elizabeth Moreira dos Santos [UNESP]
dc.contributor.authorLima, Alfredo Feio da Maia [UNESP]
dc.contributor.authorThomazini, Camila Martos [UNESP]
dc.contributor.authorMoraes, Lívia Fagundes [UNESP]
dc.contributor.authorCampoy, Gabriela [UNESP]
dc.contributor.authorGarcia, Claudia Zeferino [UNESP]
dc.contributor.authorLuna, Stelio Pacca Loureiro [UNESP]
dc.contributor.authorFagliari, José Jurandir [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2016-07-07T12:34:24Z
dc.date.available2016-07-07T12:34:24Z
dc.date.issued2011
dc.description.abstractIn this study 27 healthy dogs were spayed using cable ties as amethod of ligature of ovarian pedicles and uterine stump. Theywere divided in two groups, 18 adults (age: 11 months-7 years)and 9 young females (age: 3-9 months). Blood samples were col-lected from both groups to evaluate the protein fractions (acutephase proteins) in serum before the surgery (BS) and six monthsafter the surgery (AS) by electrophoresis in sodium dodecyl sul-phate-poliacrylamide gel. This study was approved by the AnimalExperimentation Ethics Committee (AEEC). Both groups showeda significant decrease in the results for ceruloplasmin concentra-tions six months after surgery (adults: BS 69 ± 30 mg/dL and AS35 ± 32 mg/dL, P = 0.003; young females: BS 74 ± 22 mg/dLand AS 47 ± 13 mg/dL, P = 0.017) indicating a possible relation-ship between the synthesis of this protein and the absence of 17-beta estradiol. The young females showed a significant increaseof alpha 1-antitrypsin after ovariohysterectomy (BS294 ± 113 mg/dL and AS 391 ± 125 mg/dL, P = 0.008). In thiscase, age may be responsible for this change. No statistically dif-ferences (P > 0.05) in transferrin, haptoglobin and alpha 1-acidglycoprotein concentrations were identified between groups.However, further investigations are needed to evaluate theeffects of ovariohysterectomy in acute phase protein concentra-tions over a shorter period of time.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Clínica Veterinária, Botucatu, SP, Brasil
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Ciências Agrárias e Veterinárias (FCAV), Departamento de Clínica e Cirurgia Veterinária, Jaboticabal, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Clínica Veterinária, Botucatu, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Ciências Agrárias e Veterinárias (FCAV), Departamento de Clínica e Cirurgia Veterinária, Jaboticabal, SP, Brasil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent607-607
dc.identifierhttp://dx.doi.org/10.1111/j.1939-165X.2011.00358.x
dc.identifier.citationVeterinary Clinical Pathology, v. 40, n. 4, p. 607-607, 2011.
dc.identifier.doi10.1111/j.1939-165X.2011.00358.x
dc.identifier.issn0275-6382
dc.identifier.lattes4473260410099623
dc.identifier.orcid0000-0001-5312-9076
dc.identifier.urihttp://hdl.handle.net/11449/140554
dc.language.isoeng
dc.relation.ispartofVeterinary Clinical Pathology
dc.relation.ispartofjcr0.937
dc.relation.ispartofsjr0,647
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.titleSerum protein concentrations in femaledogs submitted to ovariohysterectomy determined by means of SDS-page electro-phoresisen
dc.typeArtigo
unesp.author.lattes4473260410099623
unesp.author.orcid0000-0001-8297-6979[1]
unesp.author.orcid0000-0001-5312-9076[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentClínica Veterináriapt
unesp.departmentClínica e Cirurgia Veterinária - FCAVpt

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Artigos - Clínica Veterinária - FMVZ
Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias