Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test

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dc.contributor.authorResende, Flavia Aparecida [UNESP]
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorSantos, Lourdes Campaner dos [UNESP]
dc.contributor.authorVaranda, Eliana Aparecida [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:21:08Z
dc.date.available2014-05-20T14:21:08Z
dc.date.issued2012-05-01
dc.description.abstractThe mutagenicity of ten flavonoids was assayed by the Ames test, in Salmonella typhimurium strains TA98, TA100 and TA102, with the aim of establishing hydroxylation pattern-mutagenicity relationship profiles. The compounds assessed were: quercetin, kaempferol, luteolin, fisetin, chrysin, galangin, flavone, 3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone. In the Ames assay, quercetin acted directly and its mutagenicity increased with metabolic activation. In the presence of S9 mix, kaempferol and galangin were mutagenic in the TA98 strain and kaempferol showed signs of mutagenicity in the other strains. The absence of hydroxyl groups, as in flavone, only signs of mutagenicity were shown in strain TA102, after metabolization and, among monohydroxylated flavones (3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone), the presence of hydroxyl groups only resulted in minor changes. Luteolin and fisetin also showed signs of mutagenicity in strain TA102. Finally, chrysin, which has only two hydroxy groups, at the 5-OH and 7-OH positions, also did not induce mutagenic activity in any of the bacterial strains used, under either activation condition. All the flavonoids were tested at concentrations varying from 2.6 to 30.7 nmol/plate for galangin and 12.1 to 225.0 nmol/plate for other flavonoids. In light of the above, it is necessary to clarify the conditions and the mechanisms that mediate the biological effects of flavonoids before treating them as therapeutical agents, since some compounds can be biotransformed into more genotoxic products; as is the case for galangin, kaempferol and quercetin.en
dc.description.affiliationUNESP São Paulo State Univ, Dept Biol Sci, Fac Pharmaceut Sci Araraquara, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUNESP São Paulo State Univ, BR-11350000 Sao Vicente, SP, Brazil
dc.description.affiliationUNESP São Paulo State Univ, Dept Organ Chem, Inst Chem, BR-14800900 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP São Paulo State Univ, Dept Biol Sci, Fac Pharmaceut Sci Araraquara, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP São Paulo State Univ, BR-11350000 Sao Vicente, SP, Brazil
dc.description.affiliationUnespUNESP São Paulo State Univ, Dept Organ Chem, Inst Chem, BR-14800900 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent5255-5268
dc.identifierhttp://dx.doi.org/10.3390/molecules17055255
dc.identifier.citationMolecules. Basel: Mdpi Ag, v. 17, n. 5, p. 5255-5268, 2012.
dc.identifier.doi10.3390/molecules17055255
dc.identifier.fileWOS000304587600040.pdf
dc.identifier.issn1420-3049
dc.identifier.lattes7927877224326837
dc.identifier.lattes3538253640602977
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.urihttp://hdl.handle.net/11449/26323
dc.identifier.wosWOS:000304587600040
dc.language.isoeng
dc.publisherMdpi Ag
dc.relation.ispartofMolecules
dc.relation.ispartofjcr3.098
dc.relation.ispartofsjr0,855
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectmutagenicityen
dc.subjectAmes testen
dc.subjectflavonoidsen
dc.titleMutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Testen
dc.typeArtigo
dcterms.licensehttp://www.mdpi.com/about/openaccess
dcterms.rightsHolderMdpi Ag
unesp.author.lattes7927877224326837
unesp.author.lattes3538253640602977
unesp.author.orcid0000-0003-3032-2556[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Ciências Biológicas - FCFAR
Artigos - Ciências Biológicas - São Vicente
Artigos - Química Orgânica - IQ