Influência do tratamento periodontal nos níveis beta-defensina 3 no fluido crevicular gengival de sítios com periodontite
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Data
2023-11-30
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Universidade Estadual Paulista (Unesp)
Resumo
Os peptídeos antimicrobianos estão presentes nos tecidos periodontais e existem evidências crescentes de que estes peptídeos exercem um importante papel na resposta imune nesses tecidos. Assim, um melhor entendimento de como os peptídeos antimicrobianos são regulados nos tecidos periodontais poderá ajudar no desenvolvimento de novas estratégias preventivas, de diagnóstico e/ou terapêuticas para doença periodontal. O objetivo do estudo foi determinar a influência do tratamento periodontal n ã o cirúrgico nos níveis de beta defensina 3 (hBD 3) no fluido crevicular gengival (FGC) de sítios com periodontite. Vinte pacientes saudáveis portadores de periodontite estágio III o u IV foram incluídos no estudo. Os parâmetros clínicos periodontais foram avaliados, juntamente com a coleta de FGC de sítios doentes : um sítio com periodontite moderada (PS de 4 a 5 mm, SS, NCI≥ 4 mm) e um sítios com periodontite avançada (PS ≥ 6 mm, SS, NCI ≥ 6 mm). Em seguida, foi realizada o tratamento periodontal não cirúrgico, por meio de raspagem e alisamento radicular (RAR). Após 1 mês do término do tratamento, os parâmetros clínicos foram reavaliados, e uma nova coleta de FGC foi realizada . A qualificação dos peptídeos antimicrobianos no FGC foi feita pela técnica ELISA sanduíche. Apesar da melhora nos parâmetros clínicos, não houve mudança significativa nos níveis das hBD 3 no fluido crevicular gengival de sítios com sinais clínicos de periodontite após tratamento periodontal não cirúrgico. Entretanto, a redução de hBD 3 nos sítios com periodontite avançada após 1 mês do término do tratamento periodontal sugere que este peptídeo antimicrobiano possa ter um importante papel na resposta imune inflamatória nos tecidos periodontais.
Antimicrobial peptides are present in periodontal tissues and there is growing evidence that these peptides play an important role in the immune response in these tissues. Thus, a better understanding of how antimicrobial peptides are regulated in periodontal tissues may help in the development of new preventive, diagnostic and/or therapeutic strategies for periodontal disease. The aim of this study was to determine the influence of non-surgical periodontal treatment on beta-defensin 3 (hBD 3) levels in gingival crevicular fluid (CGF) of sites with periodontitis. Twenty healthy patients with stage III or IV periodontitis were included in the study. Periodontal clinical parameters were evaluated, along with FGC collection from diseased sites: one site with moderate periodontitis (4 to 5 mm PS, SS, NCI≥ 4 mm) and one site with advanced periodontitis (≥ 6 mm, SS, NCI ≥ 6 mm). Subsequently, non-surgical periodontal treatment was performed by means of scaling and root planing (RAR). After 1 month of the end of treatment, the clinical parameters were reassessed, and a new FGC collection was performed. The qualification of antimicrobial peptides in the FGC was performed by the sandwich ELISA technique. Despite the improvement in clinical parameters, there was no significant change in hBD 3 levels in gingival crevicular fluid from sites with clinical signs of periodontitis after non-surgical periodontal treatment. However, the reduction of hBD 3 in sites with advanced periodontitis 1 month after the end of periodontal treatment suggests that this antimicrobial peptide may play an important role in the inflammatory immune response in periodontal tissues.
Antimicrobial peptides are present in periodontal tissues and there is growing evidence that these peptides play an important role in the immune response in these tissues. Thus, a better understanding of how antimicrobial peptides are regulated in periodontal tissues may help in the development of new preventive, diagnostic and/or therapeutic strategies for periodontal disease. The aim of this study was to determine the influence of non-surgical periodontal treatment on beta-defensin 3 (hBD 3) levels in gingival crevicular fluid (CGF) of sites with periodontitis. Twenty healthy patients with stage III or IV periodontitis were included in the study. Periodontal clinical parameters were evaluated, along with FGC collection from diseased sites: one site with moderate periodontitis (4 to 5 mm PS, SS, NCI≥ 4 mm) and one site with advanced periodontitis (≥ 6 mm, SS, NCI ≥ 6 mm). Subsequently, non-surgical periodontal treatment was performed by means of scaling and root planing (RAR). After 1 month of the end of treatment, the clinical parameters were reassessed, and a new FGC collection was performed. The qualification of antimicrobial peptides in the FGC was performed by the sandwich ELISA technique. Despite the improvement in clinical parameters, there was no significant change in hBD 3 levels in gingival crevicular fluid from sites with clinical signs of periodontitis after non-surgical periodontal treatment. However, the reduction of hBD 3 in sites with advanced periodontitis 1 month after the end of periodontal treatment suggests that this antimicrobial peptide may play an important role in the inflammatory immune response in periodontal tissues.
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Palavras-chave
Beta-Defensinas, Peptídeos antimicrobianos, Doenças periodontais, Beta-Defensins, Antimicrobial Peptides, Periodontal Diseases
Como citar
Medeiros MVS. Influência do tratamento periodontal nos níveis beta-defensina 3 no fluido crevicular gengival de sítios com periodontite [Trabalho de Conclusão de Curso – Graduação em Odontologia]. Araraquara: Faculdade de Odontologia da UNESP; 2023.