Evaluations of hydroxyapatite and bioactive glass in the repair of critical size bone defects in rat calvaria

dc.contributor.authorSouza, Eduardo Quintão Manhanini [UNESP]
dc.contributor.authorCosta Klaus, Aline Evelin
dc.contributor.authorEspósito Santos, Bianca Fernanda
dc.contributor.authorCarvalho da Costa, Manuella
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorCoelho de Lima, Daniela
dc.contributor.authorFernandes, Leandro Araújo
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUndergraduate Student in Dentistry - Federal University of Alfenas (UNIFAL-MG)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionAdjunct Professor - Federal University of Alfenas (UNIFAL-MG)
dc.contributor.institutionAdjunct Professor IV - Federal University of Alfenas (UNIFAL-MG)
dc.date.accessioned2020-12-12T02:46:55Z
dc.date.available2020-12-12T02:46:55Z
dc.date.issued2020-10-01
dc.description.abstractTo overcome the morbidity of autogenous graft removal and limitations of allogeneic and xenogeneic grafts, a great interest exists in the development of biomaterials of synthetic origin. Objective: The aim of this study was to evaluate the biological behavior of a novel bioactive glass (60% SiO2- 36% CaO-4% P2O5) as bone substitute in critical calvaria defects of rats, in comparison to hydroxyapatite. Methods: Sixty male Wistar rats were divided in three groups, according to the treatment: Control Group (C) - blood clot; Hydroxyapatite (HA) - particulate hydroxyapatite (≤0,5 mm); and Bioactive Glass (BG) - particulate bioactive glass (0.04–1 mm). Results: From the intergroup analysis, it was observed that Group C presented a greater newly formed bone area (NBA) when compared to Groups HA and BG. In addition, Group HA showed higher NBA when compared to Group BG at 30 and 60 days (P < 0.05). Immunohistochemistry revealed that groups HA and BG presented high and moderate osteocalcin immunolabeling respectively. Group HA displayed a greater number of TRAP-positive cells compared to Groups C and BG at 30 and 60 days (p < 0.05). Conclusion: From these results, we can conclude that the resorption rate of hydroxyapatite is higher than the novel bioactive glass, which maintained significant higher volume until the last experimental period. Both of the tested biomaterials acted as osteoconductors during bone repair, and their physical characteristics importantly influenced this process.en
dc.description.affiliationDoctorating Student in Periodontics – São Paulo State University “Júlio de Mesquita Filho” (UNESP)
dc.description.affiliationUndergraduate Student in Dentistry - Federal University of Alfenas (UNIFAL-MG)
dc.description.affiliationDoctorating Student in Periodontics Federal University of Minas Gerais (UFMG)
dc.description.affiliationDoctor in Cell Biology Adjunct Professor - Federal University of Alfenas (UNIFAL-MG)
dc.description.affiliationProfessor of Integral Dedication to the Doctrine and Research - São Paulo State University “Júlio de Mesquita Filho” (UNESP)
dc.description.affiliationDoctor in Public Health Adjunct Professor IV - Federal University of Alfenas (UNIFAL-MG)
dc.description.affiliationDoctor in Periodontics Adjunct Professor IV - Federal University of Alfenas (UNIFAL-MG)
dc.description.affiliationUnespDoctorating Student in Periodontics – São Paulo State University “Júlio de Mesquita Filho” (UNESP)
dc.description.affiliationUnespProfessor of Integral Dedication to the Doctrine and Research - São Paulo State University “Júlio de Mesquita Filho” (UNESP)
dc.format.extent422-429
dc.identifierhttp://dx.doi.org/10.1016/j.jobcr.2020.07.014
dc.identifier.citationJournal of Oral Biology and Craniofacial Research, v. 10, n. 4, p. 422-429, 2020.
dc.identifier.doi10.1016/j.jobcr.2020.07.014
dc.identifier.issn2212-4268
dc.identifier.scopus2-s2.0-85088930671
dc.identifier.urihttp://hdl.handle.net/11449/201987
dc.language.isoeng
dc.relation.ispartofJournal of Oral Biology and Craniofacial Research
dc.sourceScopus
dc.subjectBioactive glass
dc.subjectBone regeneration
dc.subjectHydroxyapatite
dc.subjectRats
dc.titleEvaluations of hydroxyapatite and bioactive glass in the repair of critical size bone defects in rat calvariaen
dc.typeArtigo

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