Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis

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1996-10-19

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Circulating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to P. brasiliensis antigens was found in the free antibodies from PCM-CIC. This result has suggested that there were antibodies with a high affinity bound to fungus components. Dissociated CIC were fractionated in a column of Sephacryl S300 and the fractions that probably contained antigens were pooled and applied to an affinity column, prepared with mouse anti-gp43 monoclonal antibody. Using ECL-Western blotting assay two polypeptide with apparent mass of 43 and 62 kDa were found.

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adult, aged, antibody affinity, antibody detection, antigen antibody complex, clinical article, female, human, immunoblotting, male, south american blastomycosis, Adult, Aged, Animals, Antibodies, Fungal, Antibodies, Monoclonal, Antigen-Antibody Complex, Antigens, Fungal, Humans, Immunoglobulin G, Immunoglobulin M, Mice, Middle Aged, Paracoccidioidomycosis, Reference Values, Fungi, paracoccidioidomycosis brasiliensis

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Journal of Medical and Veterinary Mycology, v. 34, n. 4, p. 273-277, 1996.