Publicação:
Transfection of exogenous DNA complexed to cationic dendrimer induces alterations of bovine sperm microRNAome

dc.contributor.authorDomingues, William B.
dc.contributor.authorBlodorn, Eduardo B.
dc.contributor.authorMartins, Amanda S.W.
dc.contributor.authorDellagostin, Eduardo N.
dc.contributor.authorKomninou, Eliza R.
dc.contributor.authorHurtado, Joaquin I.
dc.contributor.authorCorcini, Carine D.
dc.contributor.authorVarela Junior, Antonio S.
dc.contributor.authorPinto, Luciano S.
dc.contributor.authorKremer, Frederico S.
dc.contributor.authorCollares, Tiago
dc.contributor.authorPinhal, Danillo [UNESP]
dc.contributor.authorGreif, Gonzalo
dc.contributor.authorRobello, Carlos
dc.contributor.authorSchneider, Augusto
dc.contributor.authorGuo, Su
dc.contributor.authorCampos, Vinicius F.
dc.contributor.institutionUniversidade Federal de Pelotas
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMontevideo
dc.contributor.institutionUniversity of California
dc.date.accessioned2020-12-12T02:14:00Z
dc.date.available2020-12-12T02:14:00Z
dc.date.issued2020-10-15
dc.description.abstractMicroRNAs have been hypothesized to be involved in the regulation of male fertility potential. The primary aim of our study was to demonstrate the effects of transfection with dendrimer nanostructure on the parameters of bovine sperm quality and to investigate whether the microRNA profile could be disturbed after cationic dendrimer-mediated exogenous DNA transfection of bovine spermatozoa. The binding of exogenous DNA was significantly increased when dendrimer-based transfection was implemented. However, cationic dendrimer transfection induced detrimental changes in the kinetics and sperm quality parameters, such as membrane integrity, acrosome reaction, and mitochondrial membrane potential, when compared to the control group. Sperm microRNA sequencing revealed 218 known and 106 novel microRNAs in the sperm samples, among which nine were dysregulated after transfection (one was upregulated and eight were downregulated), in comparison to the non-transfected sperm. All the dysregulated microRNAs were related to sperm quality and embryonic development. These results suggest that the transfection process using the dendrimer nanostructure has an impact on the quality and microRNA profile of bovine sperm.en
dc.description.affiliationLaboratório de Genômica Estrutural Programa de Pós-Graduação em Biotecnologia Centro de Desenvolvimento Tecnológico Universidade Federal de Pelotas
dc.description.affiliationLaboratório de Reprodução Animal Programa de Pós-Graduação em Veterinária Universidade Federal de Pelotas
dc.description.affiliationLaboratório de Bioinformática e Proteômica Programa de Pós-Graduação em Biotecnologia Centro de Desenvolvimento Tecnológico Universidade Federal de Pelotas
dc.description.affiliationLaboratório de Biotecnologia do Câncer Programa de Pós-Graduação em Biotecnologia Centro de Desenvolvimento Tecnológico Universidade Federal de Pelotas
dc.description.affiliationLaboratório Genômica e Evolução Molecular Departamento de Genética Instituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP)
dc.description.affiliationUnidad de Biología Molecular Institut Pasteur Montevideo
dc.description.affiliationFaculdade de Nutrição Universidade Federal de Pelotas
dc.description.affiliationDepartment of Biopharmaceutical Sciences Programs in Human Genetics and Biological Sciences University of California
dc.description.affiliationUnespLaboratório Genômica e Evolução Molecular Departamento de Genética Instituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFundação de Amparo à Pesquisa do Estado do Rio Grande do Sul: 17/2551-0000953-3
dc.description.sponsorshipIdCAPES: AUXPE 2537/2018
dc.description.sponsorshipIdFAPESP: FAPERGS-FAPESP #19/2551-0002283-2
dc.format.extent11-19
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2020.06.025
dc.identifier.citationTheriogenology, v. 156, p. 11-19.
dc.identifier.doi10.1016/j.theriogenology.2020.06.025
dc.identifier.issn0093-691X
dc.identifier.scopus2-s2.0-85087497924
dc.identifier.urihttp://hdl.handle.net/11449/200711
dc.language.isoeng
dc.relation.ispartofTheriogenology
dc.sourceScopus
dc.subjectDendrimer
dc.subjectEpigenetic
dc.subjectGene transfer
dc.subjectNon-coding RNAs
dc.subjectSpermatozoa
dc.titleTransfection of exogenous DNA complexed to cationic dendrimer induces alterations of bovine sperm microRNAomeen
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.departmentGenética - IBBpt

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