Ultrastructural analysis and residual DNA evaluation of rabbit vein scaffold
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Data
2017-09-01
Autores
Bertanha, Matheus [UNESP]
Sobreira, Marcone Lima [UNESP]
Bovolato, Ana Lívia de Carvalho [UNESP]
Rinaldi, Jaqueline de Carvalho [UNESP]
Reis, Patricia Pintor [UNESP]
Moroz, Andrei [UNESP]
de Moraes, Leonardo Nazario [UNESP]
Deffune, Elenice [UNESP]
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Resumo
Purpose: To investigate the ultrastructural characteristics and analysis of residual DNA in scaffold models, produced with decellularized vena cava in an experimental model with rabbits. Methods: Three groups were created for ultrastructural and residual DNA analysis: group 1-control, consisting of samples of vena cava in natura; group 2-SD, consisting of vein fragments submitted to 2% sodium deoxycholate decellularization by shaking (160rpm -Shaker News Brunswick Scientific*) for 1 hour at controlled temperature shaker at 37°C; group 3-SDS, consisting of vein fragments submitted to 1% sodium dodecyl sulfate decellularization under the same previous condition, for 2 hours. Results: The ultrastructural matrix of the blood vessel maintained its vintegrity after either decellularization models. The results of the two quantification methods demonstrated a significant decrease in the DNA content of the decellularized vena cava samples as compared to the control samples and, differed statistically from each other, p <0.05. Conclusion: The 2% DS protocol for vein decellularization, in this experimental model, was considered the best protocol because it presented less amount of residual DNA without causing substantial destruction of the extracellular matrix.
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Blood vessels, Peripheral arterial disease, Rabbits, Tissue engineering, Tissue scaffolds
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Acta Cirurgica Brasileira, v. 32, n. 9, p. 706-711, 2017.