Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571

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dc.contributor.authorde Oliveira, Luana Paula de Azevedo
dc.contributor.authorde Oliveira, Tiago Felipe
dc.contributor.authorContiero, Jonas [UNESP]
dc.contributor.authorCazetta, Márcia Luciana
dc.contributor.institutionUniversidade Federal do Recôncavo da Bahia
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-05-02T18:17:47Z
dc.date.available2022-05-02T18:17:47Z
dc.date.issued2016-07-01
dc.description.abstractThe factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours.en
dc.description.affiliationCentro de Ciências Agrárias Ambientais e Biológicas Universidade Federal do Recôncavo da Bahia
dc.description.affiliationUniversidade Estadual Paulista “Júlio de Mesquita Filho”
dc.description.affiliationCentro de Ciências Exatas e Tecnológicas Universidade Federal do Recôncavo da Bahia, Rua Rui Barbosa, 710
dc.description.affiliationUnespUniversidade Estadual Paulista “Júlio de Mesquita Filho”
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado da Bahia
dc.format.extent283-289
dc.identifierhttp://dx.doi.org/10.4025/actascibiolsci.v38i3.31489
dc.identifier.citationActa Scientiarum - Biological Sciences, v. 38, n. 3, p. 283-289, 2016.
dc.identifier.doi10.4025/actascibiolsci.v38i3.31489
dc.identifier.issn1807-863X
dc.identifier.issn1679-9283
dc.identifier.scopus2-s2.0-85006154526
dc.identifier.urihttp://hdl.handle.net/11449/234482
dc.language.isoeng
dc.relation.ispartofActa Scientiarum - Biological Sciences
dc.sourceScopus
dc.subjectAgricultural substrate
dc.subjectMicrobial enzymes
dc.subjectYeast
dc.titleAgave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571en
dc.titleXarope de agave como substrato para produção de inulinase por Kluyveromyces marxianus NRRL Y-7571pt
dc.typeArtigo

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Artigos - Bioquímica e Microbiologia - IBRC