qPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvae

dc.contributor.authorGiglioti, Rodrigo [UNESP]
dc.contributor.authorOliveira, Henrique Nunes de [UNESP]
dc.contributor.authorOkino, Cintia Hiromi
dc.contributor.authorSena Oliveira, Marcia Cristina de
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2018-11-29T17:54:06Z
dc.date.available2018-11-29T17:54:06Z
dc.date.issued2018-06-01
dc.description.abstractBabesia spp. are tick-transmitted intraerythrocytic apicomplexan parasites that infect wild and domestic animals. Babesia bovis and B. bigemina are endemic and responsible for enormous economic losses to the livestock industry in most of the Brazilian territory, wherein the tick Rhipicephalus microplus is the unique vector. Better understanding of epidemiology and parasite-host interactions may improve the tools for disease control and genetic management for selection of resistant animals. This study aimed to detect, quantify and measure the correlation between B. bigemina and B. bovis infection levels in bovine blood and into tick, by absolute quantification of hemoparasite DNA using qPCR. Blood bovine samples and larvae pools from 10 engorged R. microplus females were collected from each Canchim heifers (5/8 Charolais + 3/8 zebu, n = 36). All evaluated samples were positive for both Babesia species tested. Correlations of B. bovis and B. bigemina levels between cattle and tick host were 0.58 and 0.66, respectively. These high positive correlation coefficients indicate that parasitemia load in the bovine may be dependent on or may determine the parasitemia load in the ticks.en
dc.description.affiliationUniv Estadual Julio de Mesquita Filho, Unesp Jaboticabal, Sao Paulo, Brazil
dc.description.affiliationEmbrapa Pecuaria Sudeste, Rodovia Washington Luiz,Km 234,CP 339, BR-13560970 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Julio de Mesquita Filho, Unesp Jaboticabal, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipBrazilian Agricultural Research Corporation
dc.description.sponsorshipIdFAPESP: 2013/20513-5
dc.description.sponsorshipIdFAPESP: 2016/07216-7
dc.description.sponsorshipIdBrazilian Agricultural Research Corporation: SEG 02.12.02.008.00.00
dc.format.extent235-240
dc.identifierhttp://dx.doi.org/10.1007/s10493-018-0260-0
dc.identifier.citationExperimental And Applied Acarology. Dordrecht: Springer, v. 75, n. 2, p. 235-240, 2018.
dc.identifier.doi10.1007/s10493-018-0260-0
dc.identifier.fileWOS000434262500007.pdf
dc.identifier.issn0168-8162
dc.identifier.urihttp://hdl.handle.net/11449/166179
dc.identifier.wosWOS:000434262500007
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofExperimental And Applied Acarology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCorrelation
dc.subjectInfection
dc.subjectBabesiosis
dc.subjectCattle
dc.subjectTicks
dc.titleqPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvaeen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.orcid0000-0002-1700-0547[1]

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