Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization

dc.contributor.authorSantinoni, Carolina S. [UNESP]
dc.contributor.authorNeves, Adrieli P. C. [UNESP]
dc.contributor.authorAlmeida, Breno F. M. [UNESP]
dc.contributor.authorKajimoto, Natália C. [UNESP]
dc.contributor.authorPola, Natália M.
dc.contributor.authorCaliente, Eliana A. [UNESP]
dc.contributor.authorBelem, Eduarda L. G. [UNESP]
dc.contributor.authorLelis, Joilson B. [UNESP]
dc.contributor.authorFucini, Stephen E. [UNESP]
dc.contributor.authorMessora, Michel R.
dc.contributor.authorGarcia, Valdir G. [UNESP]
dc.contributor.authorBomfim, Suely R. M. [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorNagata, Maria J. H. [UNESP]
dc.contributor.institutionUNOESTE-University of Western Sao Paulo
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionPrivate Practice
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2020-12-12T02:19:36Z
dc.date.available2020-12-12T02:19:36Z
dc.date.issued2020-01-01
dc.description.abstractThe present study evaluated bone marrow aspirate (BMA) and low-level laser therapy (LLLT) on bone healing. It was created critical-size defects (CSD) of 5 mm diameter in rat calvaria of 64 rats. Animals were randomly divided into four groups: Control (blood clot), BMA (coagulated BMA), LLLT (laser irradiation and blood clot), and BMA/LLLT (laser irradiation and coagulated BMA). Euthanasia was performed at 15 or 30 days postoperative. Immunohistochemical reactions were performed to identify vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), runt-related transcription factor-2 (Runx2), bone morphogenetic protein-2 (BMP-2), osteocalcin (OCN), and osteopontin (OPN). The markers were quantified, and data were statistically analyzed. Groups BMA/LLLT and LLLT presented significantly higher VEGF expression than group control. Group BMA/LLLT presented a significantly higher expression of PCNA than all experimental groups. Groups BMA and BMA/LLLT presented significantly higher expression of BMP-2 than all experimental groups. Groups LLLT and BMA/LLLT presented significantly higher expression of OPN than groups control and BMA. Groups LLLT, BMA, and BMA/LLLT presented a significantly higher expression of OCN than group control. It can be concluded that the association of BMA and LLLT enhanced bone healing by improving expression of VEGF, PCNA, Runx2, BMP-2, OPN, and OCN.en
dc.description.affiliationDental School of Presidente Prudente Graduate Program in Dentistry (GPD-Master's Degree) UNOESTE-University of Western Sao Paulo
dc.description.affiliationDivision of Periodontics Dental School of Araçatuba Univ. Estadual Paulista–UNESP
dc.description.affiliationDivision of Clinical Surgery and Animal Reproduction Veterinary School of Araçatuba Univ. Estadual Paulista–UNESP
dc.description.affiliationDivision of Periodontics Dental School of Pelotas Federal University of Pelotas–UFPel
dc.description.affiliationPeriodontics Private Practice
dc.description.affiliationDivision of Periodontics School of Dentistry of Ribeirão Preto University of São Paulo-USP
dc.description.affiliationDivision of Histology Dental School of Araçatuba Univ. Estadual Paulista-UNESP
dc.description.affiliationUnespDivision of Periodontics Dental School of Araçatuba Univ. Estadual Paulista–UNESP
dc.description.affiliationUnespDivision of Clinical Surgery and Animal Reproduction Veterinary School of Araçatuba Univ. Estadual Paulista–UNESP
dc.description.affiliationUnespDivision of Histology Dental School of Araçatuba Univ. Estadual Paulista-UNESP
dc.identifierhttp://dx.doi.org/10.1002/jbm.a.37076
dc.identifier.citationJournal of Biomedical Materials Research - Part A.
dc.identifier.doi10.1002/jbm.a.37076
dc.identifier.issn1552-4965
dc.identifier.issn1549-3296
dc.identifier.scopus2-s2.0-85089598211
dc.identifier.urihttp://hdl.handle.net/11449/200920
dc.language.isoeng
dc.relation.ispartofJournal of Biomedical Materials Research - Part A
dc.sourceScopus
dc.subjectbone marrow
dc.subjectbone regeneration
dc.subjectimmunohistochemistry
dc.subjectlow-level laser therapy
dc.subjectmesenchymal stem cells
dc.titleBone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralizationen
dc.typeArtigo
unesp.author.orcid0000-0001-5153-2419[1]

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