Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization
dc.contributor.author | Santinoni, Carolina S. [UNESP] | |
dc.contributor.author | Neves, Adrieli P. C. [UNESP] | |
dc.contributor.author | Almeida, Breno F. M. [UNESP] | |
dc.contributor.author | Kajimoto, Natália C. [UNESP] | |
dc.contributor.author | Pola, Natália M. | |
dc.contributor.author | Caliente, Eliana A. [UNESP] | |
dc.contributor.author | Belem, Eduarda L. G. [UNESP] | |
dc.contributor.author | Lelis, Joilson B. [UNESP] | |
dc.contributor.author | Fucini, Stephen E. [UNESP] | |
dc.contributor.author | Messora, Michel R. | |
dc.contributor.author | Garcia, Valdir G. [UNESP] | |
dc.contributor.author | Bomfim, Suely R. M. [UNESP] | |
dc.contributor.author | Ervolino, Edilson [UNESP] | |
dc.contributor.author | Nagata, Maria J. H. [UNESP] | |
dc.contributor.institution | UNOESTE-University of Western Sao Paulo | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade Federal de Pernambuco (UFPE) | |
dc.contributor.institution | Private Practice | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2020-12-12T02:19:36Z | |
dc.date.available | 2020-12-12T02:19:36Z | |
dc.date.issued | 2020-01-01 | |
dc.description.abstract | The present study evaluated bone marrow aspirate (BMA) and low-level laser therapy (LLLT) on bone healing. It was created critical-size defects (CSD) of 5 mm diameter in rat calvaria of 64 rats. Animals were randomly divided into four groups: Control (blood clot), BMA (coagulated BMA), LLLT (laser irradiation and blood clot), and BMA/LLLT (laser irradiation and coagulated BMA). Euthanasia was performed at 15 or 30 days postoperative. Immunohistochemical reactions were performed to identify vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), runt-related transcription factor-2 (Runx2), bone morphogenetic protein-2 (BMP-2), osteocalcin (OCN), and osteopontin (OPN). The markers were quantified, and data were statistically analyzed. Groups BMA/LLLT and LLLT presented significantly higher VEGF expression than group control. Group BMA/LLLT presented a significantly higher expression of PCNA than all experimental groups. Groups BMA and BMA/LLLT presented significantly higher expression of BMP-2 than all experimental groups. Groups LLLT and BMA/LLLT presented significantly higher expression of OPN than groups control and BMA. Groups LLLT, BMA, and BMA/LLLT presented a significantly higher expression of OCN than group control. It can be concluded that the association of BMA and LLLT enhanced bone healing by improving expression of VEGF, PCNA, Runx2, BMP-2, OPN, and OCN. | en |
dc.description.affiliation | Dental School of Presidente Prudente Graduate Program in Dentistry (GPD-Master's Degree) UNOESTE-University of Western Sao Paulo | |
dc.description.affiliation | Division of Periodontics Dental School of Araçatuba Univ. Estadual Paulista–UNESP | |
dc.description.affiliation | Division of Clinical Surgery and Animal Reproduction Veterinary School of Araçatuba Univ. Estadual Paulista–UNESP | |
dc.description.affiliation | Division of Periodontics Dental School of Pelotas Federal University of Pelotas–UFPel | |
dc.description.affiliation | Periodontics Private Practice | |
dc.description.affiliation | Division of Periodontics School of Dentistry of Ribeirão Preto University of São Paulo-USP | |
dc.description.affiliation | Division of Histology Dental School of Araçatuba Univ. Estadual Paulista-UNESP | |
dc.description.affiliationUnesp | Division of Periodontics Dental School of Araçatuba Univ. Estadual Paulista–UNESP | |
dc.description.affiliationUnesp | Division of Clinical Surgery and Animal Reproduction Veterinary School of Araçatuba Univ. Estadual Paulista–UNESP | |
dc.description.affiliationUnesp | Division of Histology Dental School of Araçatuba Univ. Estadual Paulista-UNESP | |
dc.identifier | http://dx.doi.org/10.1002/jbm.a.37076 | |
dc.identifier.citation | Journal of Biomedical Materials Research - Part A. | |
dc.identifier.doi | 10.1002/jbm.a.37076 | |
dc.identifier.issn | 1552-4965 | |
dc.identifier.issn | 1549-3296 | |
dc.identifier.scopus | 2-s2.0-85089598211 | |
dc.identifier.uri | http://hdl.handle.net/11449/200920 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Biomedical Materials Research - Part A | |
dc.source | Scopus | |
dc.subject | bone marrow | |
dc.subject | bone regeneration | |
dc.subject | immunohistochemistry | |
dc.subject | low-level laser therapy | |
dc.subject | mesenchymal stem cells | |
dc.title | Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization | en |
dc.type | Artigo | |
unesp.author.orcid | 0000-0001-5153-2419[1] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatuba | pt |
unesp.department | Clínica, Cirurgia e Reprodução Animal - FMVA | pt |