Trypanosomatid species inDidelphis albiventrisfrom urban forest fragments

dc.contributor.authorNantes, Wesley Arruda Gimenes
dc.contributor.authorSantos, Filipe Martins
dc.contributor.authorMacedo, Gabriel Carvalho de
dc.contributor.authorBarreto, Wanessa Texeira Gomes
dc.contributor.authorGoncalves, Luiz Ricardo [UNESP]
dc.contributor.authorRodrigues, Marina Silva
dc.contributor.authorChulli, Jenyfer Valesca Monteiro
dc.contributor.authorRucco, Andreza Castro
dc.contributor.authorAssis, William de Oliveira
dc.contributor.authorPorfirio, Grasiela Edith de Oliveira
dc.contributor.authorOliveira, Carina Elisei de
dc.contributor.authorXavier, Samanta Cristina das Chagas
dc.contributor.authorHerrera, Heitor Miraglia
dc.contributor.authorJansen, Ana Maria
dc.contributor.institutionUniv Catolica Dom Bosco
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFundacao Oswaldo Cruz
dc.contributor.institutionUniv Fed deMato Grosso Sul
dc.description.abstractUrbanization results in loss of natural habitats and, consequently, reduction of richness and abundance of specialist to the detriment of generalist species. We hypothesized that a greater richness of trypanosomatid inDidelphis albiventriswould be found in fragments of urban forests in Campo Grande, Mato Grosso do Sul, Brazil, that presented a larger richness of small mammals. We used parasitological, molecular, and serological methods to detectTrypanosomaspp. infection inD. albiventris(n = 43) from forest fragments. PCR was performed with primers specific for 18S rDNA, 24S alpha rDNA, mini-chromosome satellites, and mini-exon genes. IFAT was used to detect anti-Trypanosoma cruziIgG. All hemoculture was negative. We detected trypanosomatid DNA in blood of 35% of opossum. Two opossums were seropositive forT. cruzi. The trypanosomatid species number infectingD. albiventriswas higher in the areas with greater abundance, rather than richness of small mammals. We foundD. albiventrisparasitized byT. cruziin single and co-infections withLeishmaniaspp., recently described molecular operational taxonomic unit (MOTU) named DID, andTrypanosoma lainsoni. We concluded that (i) trypanosome richness may be determined by small mammal abundance, (ii)D. albiventrisconfirmed to be bio-accumulators of trypanosomatids, and (iii)T. lainsonidemonstrated a higher host range than described up to the present.en
dc.description.affiliationUniv Catolica Dom Bosco, Posgrad Ciencias Ambientais & Sustentabilidade Ag, Av Tamandare 6000, BR-79117900 Campo Grande, MS, Brazil
dc.description.affiliationUniv Fed Mato Grosso do Sul, Posgrad Ecol & Conservacao, Campo Grande, MS, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, Sao Paulo, Brazil
dc.description.affiliationFundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Biol Tripanosomatideos, Rio De Janeiro, RJ, Brazil
dc.description.affiliationUniv Catolica Dom Bosco, Posgrad Biotecnol, Campo Grande, MS, Brazil
dc.description.affiliationUniv Fed deMato Grosso Sul, Posgrad Recursos Nat, Campo Grande, MS, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, Sao Paulo, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado deMato Grosso do Sul
dc.description.sponsorshipIdCAPES: 88887.149231/2017-00
dc.description.sponsorshipIdCNPq: 308768/2017-5
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCAPES: 88887.369261/2019-00
dc.description.sponsorshipIdFundacao de Amparo a Pesquisa do Estado deMato Grosso do Sul: FUNDECT 03/2016 PPSUS-MS 59/300.069/2017
dc.identifier.citationParasitology Research. New York: Springer, v. 120, n. 1, p. 223-231, 2021.
dc.relation.ispartofParasitology Research
dc.sourceWeb of Science
dc.subjectTrypanosoma cruzi
dc.subjectTrypanosoma lainsoni
dc.titleTrypanosomatid species inDidelphis albiventrisfrom urban forest fragmentsen