Publicação:
Identification of a Specific miRNA Profile in HIV-Exposed Seronegative Individuals

dc.contributor.authorYahyaei, Sara
dc.contributor.authorBiasin, Mara
dc.contributor.authorSaulle, Irma
dc.contributor.authorGnudi, Federica
dc.contributor.authorDe Luca, Mariacristina
dc.contributor.authorTasca, Karen I. [UNESP]
dc.contributor.authorTrabattoni, Daria
dc.contributor.authorLo Caputo, Sergio
dc.contributor.authorMazzotta, Francesco
dc.contributor.authorClerici, Mario
dc.contributor.institutionUniv Milan
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionS Maria Annunziata Hosp Florence
dc.contributor.institutionIRCCS
dc.date.accessioned2018-11-26T17:06:14Z
dc.date.available2018-11-26T17:06:14Z
dc.date.issued2016-09-01
dc.description.abstractObjective:MicroRNAs (miRNAs) are small noncoding RNAs involved in the posttranscriptional regulation of gene expression that play important roles in viral infections. Alterations of specific miRNAs are described in HIV infection, suggesting a role for miRNAs in pathogenesis of this disease. We verified whether a particular miRNA signature could be identified in natural resistance to HIV-1.Methods:Expression level of 84 miRNAs was analyzed by RT-qPCR in plasma and unstimulated peripheral blood mononuclear cell (PBMC) of 30 seronegative individuals repeatedly exposed to HIV-1 (HESN), 30 HIV seropositive subjects (HIV+), and 30 healthy controls (HC). Results were confirmed by individual RT-qPCR in in vitro HIV-1-infected PBMC and in their cell culture medium. Dicer and Drosha expression was analyzed in basal PBMC.Results:Whereas Dicer and Drosha expression was comparable in HESN, HIV+ and HC, several miRNAs were upregulated both in HESN and HIV+ compared with HC. Furthermore, miRNA-29a and miR-223 were upregulated in both unstimulated PBMC and plasma of HESN alone; their expression was reduced upon in vitro HIV-1 infection of HESN PBMC indicating that, upon infection, they are secreted in the extracellular milieu. These results were confirmed by individual qPCR.Conclusions:Our studies demonstrate that HIV-1 exposure modifies miRNAs expression even in the absence of productive infection. Because those miRNAs that are specifically increased only in HESN have been known to reduce HIV-1 replication, their modulation could represent an important mechanism in resistance to HIV-1 infection.en
dc.description.affiliationUniv Milan, Dept Biomed & Clin Sci L Sacco, Milan, Italy
dc.description.affiliationUniv Estadual Paulista, Dept Trop Dis, Botucatu, SP, Brazil
dc.description.affiliationS Maria Annunziata Hosp Florence, Infect Dis Unit, Florence, Italy
dc.description.affiliationUniv Milan, Dept Pathophysiol & Transplantat, I-20122 Milan, Italy
dc.description.affiliationIRCCS, Don Gnocchi Fdn, ONLUS, Milan, Italy
dc.description.affiliationUnespUniv Estadual Paulista, Dept Trop Dis, Botucatu, SP, Brazil
dc.format.extent11-19
dc.identifierhttp://dx.doi.org/10.1097/QAI.0000000000001070
dc.identifier.citationJaids-journal Of Acquired Immune Deficiency Syndromes. Philadelphia: Lippincott Williams & Wilkins, v. 73, n. 1, p. 11-19, 2016.
dc.identifier.doi10.1097/QAI.0000000000001070
dc.identifier.issn1525-4135
dc.identifier.urihttp://hdl.handle.net/11449/161933
dc.identifier.wosWOS:000383563500004
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofJaids-journal Of Acquired Immune Deficiency Syndromes
dc.relation.ispartofsjr2,368
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectHIV-1
dc.subjectHESN
dc.subjectmiRNA
dc.subjectresistance to infection
dc.titleIdentification of a Specific miRNA Profile in HIV-Exposed Seronegative Individualsen
dc.typeArtigo
dcterms.rightsHolderLippincott Williams & Wilkins
dspace.entity.typePublication
unesp.author.orcid0000-0001-9535-3359[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.departmentDoenças Tropicais e Diagnósticos por Imagem - FMBpt

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