Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials
dc.contributor.author | Ribeiro, Juliana Silva | |
dc.contributor.author | Bordini, Ester Alves Ferreira [UNESP] | |
dc.contributor.author | Pereira, Gabriel Kalil Rocha | |
dc.contributor.author | Polasani, Rohitha Rao | |
dc.contributor.author | Squarize, Cristiane Helena | |
dc.contributor.author | Kantorski, Karla Zanini | |
dc.contributor.author | Valandro, Luiz Felipe | |
dc.contributor.author | Bottino, Marco Cícero | |
dc.contributor.institution | University of Michigan School of Dentistry | |
dc.contributor.institution | Federal University of Pelotas | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | Universidade Federal de Sergipe (UFS) | |
dc.date.accessioned | 2022-04-28T19:49:41Z | |
dc.date.available | 2022-04-28T19:49:41Z | |
dc.date.issued | 2022-01-01 | |
dc.description.abstract | Objectives: To modify the surface of denture base material by coating it with cinnamon-laden nanofibers to reduce Candida albicans (C. albicans) adhesion and/or proliferation. Materials and methods: Heat-cured poly(methyl methacrylate) (PMMA) specimens were processed and coated, or not, with cinnamon-laden polymeric nanofibers (20 or 40 wt.% of cinnamon relative to the total polymer weight). Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) analyses of the nanofibers were performed. Antifungal activity was assessed through agar diffusion and colony-forming unit (CFU/mL) assays. Representative SEM morphological analysis was carried out to observe the presence/absence of C. albicans on the fibers. Alamar blue assay was used to determine cell toxicity. Analysis of variance and the Tukey’s test were used to analyze the data (α = 0.05). Results: SEM imaging revealed nanofibers with adequate (i.e., bead-free) morphological characteristics and uniform microstructure. FTIR confirmed cinnamon incorporation. The cinnamon-laden nanofibers led to growth inhibition of C. albicans. Viable fungal counts support a significant reduction on CFU/mL also directly related to cinnamon concentration (40 wt.%: mean log 6.17 CFU/mL < 20 wt.%: mean log 7.12 CFU/mL), which agrees with the SEM images. Cinnamon-laden nanofibers at 40 wt.% led to increased cell death. Conclusions: The deposition of 20 wt.% cinnamon-laden nanofibers onto PMMA surfaces led to a significant reduction of the adhesive and/or proliferative ability of C. albicans, while maintaining epithelial cells’ viability. Clinical relevance: The high recurrence rates of denture stomatitis are associated with patient non-adherence to treatments and contaminated prostheses use. Here, we provide the non-patients’ cooperation sensible method, which possesses antifungal action, hence improving treatment effectiveness. | en |
dc.description.affiliation | Department of Cariology Restorative Sciences and Endodontics University of Michigan School of Dentistry, 1011 N. University (Room 5223) | |
dc.description.affiliation | Department of Restorative Dentistry School of Dentistry Federal University of Pelotas, RS | |
dc.description.affiliation | Department of Physiology and Pathology University Estadual Paulista – UNESP, SP | |
dc.description.affiliation | Post-Graduate Program in Oral Science Faculty of Dentistry Federal University of Santa Maria (UFSM), Rio Grande Do Sul State | |
dc.description.affiliation | Department of Periodontics and Oral Medicine University of Michigan School of Dentistry | |
dc.description.affiliationUnesp | Department of Physiology and Pathology University Estadual Paulista – UNESP, SP | |
dc.identifier | http://dx.doi.org/10.1007/s00784-021-04341-5 | |
dc.identifier.citation | Clinical Oral Investigations. | |
dc.identifier.doi | 10.1007/s00784-021-04341-5 | |
dc.identifier.issn | 1436-3771 | |
dc.identifier.issn | 1432-6981 | |
dc.identifier.scopus | 2-s2.0-85123060681 | |
dc.identifier.uri | http://hdl.handle.net/11449/223282 | |
dc.language.iso | eng | |
dc.relation.ispartof | Clinical Oral Investigations | |
dc.source | Scopus | |
dc.subject | Candida albicans | |
dc.subject | Cinnamomum verum | |
dc.subject | Denture stomatitis | |
dc.subject | Electrospinning | |
dc.subject | Nanofibers | |
dc.title | Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials | en |
dc.type | Artigo |