Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials

dc.contributor.authorRibeiro, Juliana Silva
dc.contributor.authorBordini, Ester Alves Ferreira [UNESP]
dc.contributor.authorPereira, Gabriel Kalil Rocha
dc.contributor.authorPolasani, Rohitha Rao
dc.contributor.authorSquarize, Cristiane Helena
dc.contributor.authorKantorski, Karla Zanini
dc.contributor.authorValandro, Luiz Felipe
dc.contributor.authorBottino, Marco Cícero
dc.contributor.institutionUniversity of Michigan School of Dentistry
dc.contributor.institutionFederal University of Pelotas
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de Sergipe (UFS)
dc.description.abstractObjectives: To modify the surface of denture base material by coating it with cinnamon-laden nanofibers to reduce Candida albicans (C. albicans) adhesion and/or proliferation. Materials and methods: Heat-cured poly(methyl methacrylate) (PMMA) specimens were processed and coated, or not, with cinnamon-laden polymeric nanofibers (20 or 40 wt.% of cinnamon relative to the total polymer weight). Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) analyses of the nanofibers were performed. Antifungal activity was assessed through agar diffusion and colony-forming unit (CFU/mL) assays. Representative SEM morphological analysis was carried out to observe the presence/absence of C. albicans on the fibers. Alamar blue assay was used to determine cell toxicity. Analysis of variance and the Tukey’s test were used to analyze the data (α = 0.05). Results: SEM imaging revealed nanofibers with adequate (i.e., bead-free) morphological characteristics and uniform microstructure. FTIR confirmed cinnamon incorporation. The cinnamon-laden nanofibers led to growth inhibition of C. albicans. Viable fungal counts support a significant reduction on CFU/mL also directly related to cinnamon concentration (40 wt.%: mean log 6.17 CFU/mL < 20 wt.%: mean log 7.12 CFU/mL), which agrees with the SEM images. Cinnamon-laden nanofibers at 40 wt.% led to increased cell death. Conclusions: The deposition of 20 wt.% cinnamon-laden nanofibers onto PMMA surfaces led to a significant reduction of the adhesive and/or proliferative ability of C. albicans, while maintaining epithelial cells’ viability. Clinical relevance: The high recurrence rates of denture stomatitis are associated with patient non-adherence to treatments and contaminated prostheses use. Here, we provide the non-patients’ cooperation sensible method, which possesses antifungal action, hence improving treatment effectiveness.en
dc.description.affiliationDepartment of Cariology Restorative Sciences and Endodontics University of Michigan School of Dentistry, 1011 N. University (Room 5223)
dc.description.affiliationDepartment of Restorative Dentistry School of Dentistry Federal University of Pelotas, RS
dc.description.affiliationDepartment of Physiology and Pathology University Estadual Paulista – UNESP, SP
dc.description.affiliationPost-Graduate Program in Oral Science Faculty of Dentistry Federal University of Santa Maria (UFSM), Rio Grande Do Sul State
dc.description.affiliationDepartment of Periodontics and Oral Medicine University of Michigan School of Dentistry
dc.description.affiliationUnespDepartment of Physiology and Pathology University Estadual Paulista – UNESP, SP
dc.identifier.citationClinical Oral Investigations.
dc.relation.ispartofClinical Oral Investigations
dc.subjectCandida albicans
dc.subjectCinnamomum verum
dc.subjectDenture stomatitis
dc.titleNovel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materialsen