Zoledronic Acid Inhibits Human Osteoblast Activities

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dc.contributor.authorBasso, Fernanda G.
dc.contributor.authorSilveira Turrioni, Ana Paula [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorSouza Costa, Carlos A. de [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:07:05Z
dc.date.available2014-12-03T13:07:05Z
dc.date.issued2013-01-01
dc.description.abstractBackground: Bisphosphonates are potent inhibitors of bone resorption. These kinds of drugs, which are used for the treatment of osteolytic diseases, have been associated with the occurrence of oral osteonecrosis, especially in patients over 60 years old. Current studies have demonstrated that the cytotoxic effects of bisphosphonates on osteoblasts play an important role in oral osteonecrosis development. Objective:The aim of this study was to evaluate the effect of long-term application of a highly potent bisphosphonate - zoledronic acid (ZA) - on human osteoblasts in vitro. Methods: Human osteoblasts (MG63 cell line) were seeded for 72 h in wells of 24-well plates. The Dulbecco's modified Eagle's medium (DMEM) was then replaced by culture medium without fetal bovine serum (FBS), and the cells were incubated for an additional 24 h, after which ZA was added to the DMEM without FBS and incubated in contact with osteoblasts for 7, 14 or 21 days. Cell viability (CV), total protein production (TPP), alkaline phosphatase (ALP) activity, mineral nodule formation (MNF), and gene expression of ALP and osteocalcin (OCN), as well as cell morphology by scanning electronic microscopy, were evaluated. Data were statistically analyzed by Kruskal-Wallis and Mann-Whitney tests, with a significance level of 5%. Results:The cytotoxic effects of ZA on osteoblasts were characterized by reduction of CV, TPP, ALP and MNF production. In addition, ZA MNF caused a decrease in gene expression of ALP and OCN, as well as intense cell morphology alterations. All these negative effects of ZA were concentration and period dependent. Conclusion: Both concentrations of ZA (1 and 5 mu m) caused cytotoxic effects to osteoblasts which reduced the production and expression of proteins that play an important role in bone matrix synthesis and mineralization. (C) 2013 S. Karger AG, Baselen
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Piracicaba Sch Dent, Piracicaba, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 09/54722-1
dc.description.sponsorshipIdFAPESP: 09/52326-1
dc.description.sponsorshipIdCNPq: 301291/2010-1
dc.format.extent534-541
dc.identifierhttp://dx.doi.org/10.1159/000351194
dc.identifier.citationGerontology. Basel: Karger, v. 59, n. 6, p. 534-541, 2013.
dc.identifier.doi10.1159/000351194
dc.identifier.issn0304-324X
dc.identifier.lattes4517484241515548
dc.identifier.urihttp://hdl.handle.net/11449/111235
dc.identifier.wosWOS:000326135900007
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofGerontology
dc.relation.ispartofjcr3.532
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBisphosphonatesen
dc.subjectOsteonecrosisen
dc.subjectOsteoblastsen
dc.subjectCytotoxicityen
dc.titleZoledronic Acid Inhibits Human Osteoblast Activitiesen
dc.typeArtigo
dcterms.licensehttp://www.karger.com/Services/RightsPermissions
dcterms.rightsHolderKarger
unesp.author.lattes4517484241515548[4]
unesp.author.orcid0000-0002-7455-6867[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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