Transcriptional changes involved in kumquat (Fortunella spp) defense response to Xanthomonas citri subsp. citri in early stages of infection

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dc.contributor.authorGiraldo – González, Jhon Jairo
dc.contributor.authorde Souza Carvalho, Flávia Maria [UNESP]
dc.contributor.authorFerro, Jesus Aparecido [UNESP]
dc.contributor.authorHerai, Roberto Hirochi
dc.contributor.authorChaves Bedoya, Giovanni
dc.contributor.authorRodas Mendoza, Elkin Fernando
dc.contributor.institutionGrupo de Investigación en Fitobioquímica y Biología Molecular “Fitobiomol”
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionGraduate Program in Health Sciences. School of Medicine
dc.date.accessioned2022-04-29T08:36:07Z
dc.date.available2022-04-29T08:36:07Z
dc.date.issued2021-12-01
dc.description.abstractXanthomonas citri subsp. citri (Xcc), the causal agent of type A canker, differentially affects all commercial varieties of citrus plants. Among them, Fortunella spp (kumquat) have higher tolerance to disease development, although the molecular mechanisms involved in their defense are not fully understood. In this study, using RNA-seq approach, the transcriptional responses of kumquat leaves at 24 h after water (controls) and Xcc inoculation were analyzed. A total of 1439 Differentially expressed genes (DEG) with statistical significance (p-value<0.025) were identified, with 444 being upregulated. These genes were found to be involved in pathogen recognition, cell wall remodeling and reinforcement, lignin biosynthesis, reactive oxygen species (ROS) production, pathogenesis-related proteins (PR) and biosynthesis of secondary metabolites including phenylpropanoids, terpenoids and alkaloids. Genes related to disease resistance (R genes) and salicylic acid (SA)-dependent systemic acquired resistance (SAR) were also induced. In turn, the 995 downregulated genes were mainly associated with photosynthesis, photorespiration, chlorophyll synthesis and cell growth. This suggest that, once the pathogen is detected, the plant generates a strong oxidative burst and its cellular machinery is directed towards the synthesis of secondary metabolites and defense proteins while its own growth is inhibited. Overall, these transcriptional changes provide valuable information about the molecular basis of the defense in kumquat plants, which may be useful in the design of new control methods for citrus canker.en
dc.description.affiliationUniversidad Francisco de Paula Santander Facultad de Ciencias Básicas Grupo de Investigación en Fitobioquímica y Biología Molecular “Fitobiomol”, Cúcuta, Norte de Santander
dc.description.affiliationUniversidade Estadual Paulista (UNESP) Departamento de Tecnologia. Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Jaboticabal
dc.description.affiliationPontifícia Universidade Católica do Paraná (PUCPR) Graduate Program in Health Sciences. School of Medicine
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) Departamento de Tecnologia. Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Jaboticabal
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação Araucária
dc.description.sponsorshipIdCAPES: 3371/2013
dc.description.sponsorshipIdFundação Araucária: CP09/2016
dc.identifierhttp://dx.doi.org/10.1016/j.pmpp.2021.101729
dc.identifier.citationPhysiological and Molecular Plant Pathology, v. 116.
dc.identifier.doi10.1016/j.pmpp.2021.101729
dc.identifier.issn1096-1178
dc.identifier.issn0885-5765
dc.identifier.scopus2-s2.0-85118338220
dc.identifier.urihttp://hdl.handle.net/11449/229819
dc.language.isoeng
dc.relation.ispartofPhysiological and Molecular Plant Pathology
dc.sourceScopus
dc.subjectCitrus canker
dc.subjectDifferentially expressed genes (DEG)
dc.subjectPlant immunity
dc.subjectPlant-pathogen interaction
dc.subjectRNA-seq
dc.titleTranscriptional changes involved in kumquat (Fortunella spp) defense response to Xanthomonas citri subsp. citri in early stages of infectionen
dc.typeArtigo
unesp.author.orcid0000-0001-7665-7840[1]
unesp.author.orcid0000-0001-9759-5212[2]
unesp.author.orcid0000-0003-1013-614X[5]

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