Cytotoxic effects of white-MTA and MTA-Bio cements on odontoblast-like cells (MDPC-23)

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dc.contributor.authorLessa, Fernanda Campos Rosetti [UNESP]
dc.contributor.authorAranha, Andreza Maria Fábio [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorCosta, Carlos Alberto de Souza [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:24:36Z
dc.date.available2014-05-27T11:24:36Z
dc.date.issued2010-01-01
dc.description.abstractThis study evaluated the cytotoxic effects of 2 mineral trioxide aggregate (MTA) cements - White-MTA-Angelus and a new formulation, MTA-Bio - on odontoblast-like cell (MDPC-23) cultures. Twenty-four disc-shaped (2 mm diameter x 2 mm thick) specimens were fabricated from each material and immersed individually in wells containing 1 mL of DMEM culture medium for either 24 h or 7 days to obtain extracts, giving rise to 4 groups of 12 specimens each: G1 - White-MTA/24 h; G2 - White-MTA/7 days; G3 - MTA-Bio/24 h; and G4 - MTA-Bio/7 days. Plain culture medium (DMEM) was used as a negative control (G5). Cells at 30,000 cells/cm 2 concentration were seeded in the wells of 24-well plates and incubated in a humidified incubator with 5% CO 2 and 95% air at 37°C for 72 h. After this period, the culture medium of each well was replaced by 1 mL of extract (or plain DMEM in the control group) and the cells were incubated for additional 2 h. Cell metabolism was evaluated by the MTT assay and the data were analyzed statistically by ANOVA and Tukey's test (α=0.05). Cell morphology and the surface of representative MTA specimens of each group were examined by scanning electron microscopy. There was no statistically significant difference (p>0.05) between G1 and G2 or between G3 and G4. No significant difference (p>0.05) was found between the experimental and control groups either. Similar cell organization and morphology were observed in all groups, regardless of the storage periods. However, the number of cells observed in the experimental groups decreased compared to the control group. MTA-Bio presented irregular surface with more porosities than White-MTA. In conclusion, White-MTA and MTA-Bio presented low cytotoxic effects on odontoblast-like cell (MDPC-23) cultures.en
dc.description.affiliationDepartment of Orthodontics and Pediatric Dentistry Araraquara Dental School São Paulo State University, Araraquara, SP
dc.description.affiliationLaboratory of General Pathology and Biomaterials, Department of Physiology and Pathology Araraquara Dental School São Paulo State University, Araraquara, SP
dc.description.affiliationUnespDepartment of Orthodontics and Pediatric Dentistry Araraquara Dental School São Paulo State University, Araraquara, SP
dc.description.affiliationUnespLaboratory of General Pathology and Biomaterials, Department of Physiology and Pathology Araraquara Dental School São Paulo State University, Araraquara, SP
dc.format.extent24-31
dc.identifierhttp://dx.doi.org/10.1590/S0103-64402010000100004
dc.identifier.citationBrazilian Dental Journal, v. 21, n. 1, p. 24-31, 2010.
dc.identifier.doi10.1590/S0103-64402010000100004
dc.identifier.fileS0103-64402010000100004.pdf
dc.identifier.issn0103-6440
dc.identifier.issn1806-4760
dc.identifier.lattes4517484241515548
dc.identifier.scieloS0103-64402010000100004
dc.identifier.scopus2-s2.0-77955605648
dc.identifier.urihttp://hdl.handle.net/11449/71518
dc.language.isoeng
dc.relation.ispartofBrazilian Dental Journal
dc.relation.ispartofsjr0,476
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiomaterials
dc.subjectCell culture
dc.subjectCytotoxicity
dc.subjectMTA
dc.subjectOdontoblasts
dc.subjectaluminum derivative
dc.subjectcalcium derivative
dc.subjectcoloring agent
dc.subjectdiagnostic agent
dc.subjectmineral trioxide aggregate
dc.subjectMTA Bio
dc.subjectoxide
dc.subjectroot canal filling material
dc.subjectsilicate
dc.subjectsuccinate dehydrogenase
dc.subjecttetrazolium
dc.subjectthiazole derivative
dc.subjectthiazolyl blue
dc.subjecttooth cement
dc.subjectcell count
dc.subjectcell line
dc.subjectcell shape
dc.subjectcell survival
dc.subjectchemistry
dc.subjectcomparative study
dc.subjectculture medium
dc.subjectculture technique
dc.subjectdrug combination
dc.subjectdrug effect
dc.subjecthuman
dc.subjectmaterials testing
dc.subjectodontoblast
dc.subjectporosity
dc.subjectscanning electron microscopy
dc.subjectspectrophotometry
dc.subjectsurface property
dc.subjecttemperature
dc.subjecttime
dc.subjectAluminum Compounds
dc.subjectCalcium Compounds
dc.subjectCell Count
dc.subjectCell Culture Techniques
dc.subjectCell Line
dc.subjectCell Shape
dc.subjectCell Survival
dc.subjectColoring Agents
dc.subjectCulture Media
dc.subjectDental Cements
dc.subjectDrug Combinations
dc.subjectHumans
dc.subjectMaterials Testing
dc.subjectMicroscopy, Electron, Scanning
dc.subjectOxides
dc.subjectPorosity
dc.subjectRoot Canal Filling Materials
dc.subjectSilicates
dc.subjectSpectrophotometry
dc.subjectSuccinate Dehydrogenase
dc.subjectSurface Properties
dc.subjectTemperature
dc.subjectTetrazolium Salts
dc.subjectThiazoles
dc.subjectTime Factors
dc.titleCytotoxic effects of white-MTA and MTA-Bio cements on odontoblast-like cells (MDPC-23)en
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bdj/paboutj.htm
unesp.author.lattes4517484241515548[4]
unesp.author.orcid0000-0002-7455-6867[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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