Genome-Wide Analysis of Differentially Expressed Genes During the Early Stages of Tomato Infection by a Potyvirus

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dc.contributor.authorAlfenas-Zerbini, Poliane
dc.contributor.authorMaia, Ivan de Godoy [UNESP]
dc.contributor.authorFavaro, Regiane D. [UNESP]
dc.contributor.authorCascardo, Julio C. M.
dc.contributor.authorBrommonschenkel, Sergio H.
dc.contributor.authorZerbini, Francisco Murilo
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Santa Cruz (UESC)
dc.date.accessioned2014-05-20T13:50:22Z
dc.date.available2014-05-20T13:50:22Z
dc.date.issued2009-03-01
dc.description.abstractPlant responses against pathogens cause up-and downward shifts in gene expression. To identify differentially expressed genes in a plant-virus interaction, susceptible tomato plants were inoculated with the potyvirus Pepper yellow mosaic virus (PepYMV) and a subtractive library was constructed from inoculated leaves at 72 h after inoculation. Several genes were identified as upregulated, including genes involved in plant defense responses (e. g., pathogenesis-related protein 5), regulation of the cell cycle (e. g., cytokinin-repressed proteins), signal transduction (e. g., CAX-interacting protein 4, SNF1 kinase), transcriptional regulators (e. g., WRKY and SCARECROW transcription factors), stress response proteins (e. g., Hsp90, DNA-J, 20S proteasome alpha subunit B, translationally controlled tumor protein), ubiquitins (e. g., polyubiquitin, ubiquitin activating enzyme 2), among others. Downregulated genes were also identified, which likewise display identity with genes involved in several metabolic pathways. Differential expression of selected genes was validated by macroarray analysis and quantitative real-time polymerase chain reaction. The possible roles played by some of these genes in the viral infection cycle are discussed.en
dc.description.affiliationUniversidade Federal de Viçosa (UFV), Dept Fitopatol BIOAGRO, BR-36570000 Vicosa, MG, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Genet, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Santa Cruz, Dep Biol & Genet Mol, BR-45662000 Ilheus, BA, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Genet, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipAgricultural Technology Development Project for Brazil
dc.description.sponsorshipFoundation of Minas Gerais State
dc.description.sponsorshipIdAgricultural Technology Development Project for Brazil: 052/01
dc.description.sponsorshipIdFoundation of Minas Gerais State: CAG336/06
dc.format.extent352-361
dc.identifierhttp://dx.doi.org/10.1094/MPMI-22-3-0352
dc.identifier.citationMolecular Plant-microbe Interactions. St Paul: Amer Phytopathological Soc, v. 22, n. 3, p. 352-361, 2009.
dc.identifier.doi10.1094/MPMI-22-3-0352
dc.identifier.issn0894-0282
dc.identifier.lattes8649222099176162
dc.identifier.urihttp://hdl.handle.net/11449/17978
dc.identifier.wosWOS:000263204600012
dc.language.isoeng
dc.publisherAmer Phytopathological Soc
dc.relation.ispartofMolecular Plant-Microbe Interactions
dc.relation.ispartofjcr3.588
dc.relation.ispartofsjr1,935
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleGenome-Wide Analysis of Differentially Expressed Genes During the Early Stages of Tomato Infection by a Potyvirusen
dc.typeArtigo
dcterms.licensehttp://apsjournals.apsnet.org/page/terms.jsp
dcterms.rightsHolderAmer Phytopathological Soc
unesp.author.lattes8649222099176162
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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