Low-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilm

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dc.contributor.authorFigueira, Leandro W. [UNESP]
dc.contributor.authorPanariello, Beatriz H. D.
dc.contributor.authorKoga-Ito, Cristiane Y. [UNESP]
dc.contributor.authorDuarte, Simone
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionIndiana University School of Dentistry
dc.date.accessioned2021-06-25T10:20:09Z
dc.date.available2021-06-25T10:20:09Z
dc.date.issued2021-01-02
dc.description.abstractThis study aimed to determine how low-temperature plasma (LTP) treatment affects single-and multi-species biofilms formed by Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii formed on hydroxyapatite discs. LTP was produced by argon gas using the kIN-Pen09™ (Leibniz Institute for Plasma Science and Technology, INP, Greifswald, Germany). Biofilms were treated at a 10 mm distance from the nozzle of the plasma device to the surface of the biofilm per 30 s, 60 s, and 120 s. A 0.89% saline solution and a 0.12% chlorhexidine solution were used as negative and positive controls, respectively. Argon flow at three exposure times (30 s, 60 s, and 120 s) was also used as control. Biofilm viability was analyzed by colony-forming units (CFU) recovery and confocal laser scanning microscopy. Multispecies biofilms presented a reduction in viability (log10 CFU/mL) for all plasma-treated samples when compared to both positive and negative controls (p < 0.0001). In single-species biofilms formed by either S. mutans or S. sanguinis, a significant reduction in all exposure times was observed when compared to both positive and negative controls (p < 0.0001). For single-species biofilms formed by S. gordonii, the results indicate total elimination of S. gordonii for all exposure times. Low exposure times of LTP affects single-and multi-species cariogenic biofilms, which indicates that the treatment is a promising source for the development of new protocols for the control of dental caries.en
dc.description.affiliationInstitute of Science and Technology São Paulo State University UNESP
dc.description.affiliationDepartment of Cariology Operative Dentistry and Dental Public Health Indiana University School of Dentistry
dc.description.affiliationUnespInstitute of Science and Technology São Paulo State University UNESP
dc.description.sponsorshipNational Institutes of Health
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdNational Institutes of Health: 1R21DE028929-01
dc.description.sponsorshipIdFAPESP: 2019/01676-4
dc.description.sponsorshipIdFAPESP: 2019/05856-7
dc.format.extent1-12
dc.identifierhttp://dx.doi.org/10.3390/app11020570
dc.identifier.citationApplied Sciences (Switzerland), v. 11, n. 2, p. 1-12, 2021.
dc.identifier.doi10.3390/app11020570
dc.identifier.issn2076-3417
dc.identifier.lattes6543563161403421
dc.identifier.orcid0000-0002-2416-2173
dc.identifier.scopus2-s2.0-85099256771
dc.identifier.urihttp://hdl.handle.net/11449/205720
dc.language.isoeng
dc.relation.ispartofApplied Sciences (Switzerland)
dc.sourceScopus
dc.subjectAntibacterial
dc.subjectDental caries
dc.subjectStreptococcus gordonii
dc.subjectStreptococcus mutans
dc.subjectStreptococcus sanguinis
dc.titleLow-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilmen
dc.typeArtigo
unesp.author.lattes6543563161403421[3]
unesp.author.orcid0000-0002-2416-2173[3]

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