Phenotypic and genotypic characterization of Rhodococcus equi isolated from sputum

dc.contributor.authorda Silva, Paulo
dc.contributor.authorBarreto Santos, Adolfo Carlos [UNESP]
dc.contributor.authorSato, Daisy Nakamura
dc.contributor.authorSilva, Jaqueline Otero
dc.contributor.authorCazentini Medeiros, Marta Ines
dc.contributor.authorMachado Carneiro, Ana Maria
dc.contributor.authorde Andrade Leite, Sergio Roberto
dc.contributor.authorLeite, Clarice Queico Fujimura [UNESP]
dc.contributor.institutionInstituto Adolfo Lutz (IAL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:24:21Z
dc.date.available2014-05-20T13:24:21Z
dc.date.issued2012-09-01
dc.description.abstractIntroduction: Rhodococcus equi is an opportunistic pathogen, causing rhodococcosis, a condition that can be confused with tuberculosis. Often, without identifying M. tuberculosis, physicians initiate empiric treatment for tuberculosis. R. equi and M. tuberculosis have different susceptibility to drugs. Identification of R. equi is based on a variety of phenotypic, chromatographic, and genotypic characteristics.Objective: This study aimed to characterize bacterial isolates from sputum samples suggestive of R. equi.Methods: The phenotypic identification included biochemical assays; thin-layer chromatography (TLC) and polymerase chain reaction (PCR) were used for genotypic identification.Results: Among 78 Gram-positive and partially acid-fast bacilli isolated from the sputum of tuberculosis-suspected patients, 51 were phenotypically and genotypically characterized as R. equi based on literature data. Mycolic acid analysis showed that all suspected R. equi had compounds with a retention factor (R-f) between 0.4-0.5. Genotypic characterization indicated the presence of the choE gene 959 bp fragments in 51 isolates CAMP test positive. Twenty-two CAMP test negative isolates were negative for the choE gene. Five isolates presumptively identified as R. equi, CAMP test positive, were choE gene negative, and probably belonged to other bacterial species.Conclusions: The phenotypic and molecular techniques used constitute a good methodological tool to identify R. equi. (C) 2012 Elsevier Editora All rights reserved.en
dc.description.affiliationAdolfo Lutz Inst, Nucleo Ciencias Biomed, Ctr Lab Reg IAL NCB CLR 6, BR-14085410 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho UNESP, Sch Pharmaceut Sci, Lab Micobacterias Prof Dr Hugo David, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho UNESP, Sch Pharmaceut Sci, Lab Micobacterias Prof Dr Hugo David, Araraquara, SP, Brazil
dc.format.extent409-415
dc.identifierhttp://dx.doi.org/10.1016/j.bjid.2012.08.009
dc.identifier.citationBrazilian Journal of Infectious Diseases. Salvador: Contexto, v. 16, n. 5, p. 409-415, 2012.
dc.identifier.doi10.1016/j.bjid.2012.08.009
dc.identifier.fileWOS000310088400001.pdf
dc.identifier.issn1413-8670
dc.identifier.lattes2114570774349859
dc.identifier.urihttp://hdl.handle.net/11449/7515
dc.identifier.wosWOS:000310088400001
dc.language.isoeng
dc.publisherContexto
dc.relation.ispartofBrazilian Journal of Infectious Diseases
dc.relation.ispartofjcr2.083
dc.relation.ispartofsjr0,817
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectPolymerase chain reactionen
dc.subjectRhodococcus equien
dc.subjectMycolic acidsen
dc.titlePhenotypic and genotypic characterization of Rhodococcus equi isolated from sputumen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderContexto
unesp.author.lattes2114570774349859
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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