Heparin is biocompatible and can induce differentiation of human dental pulp cells

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Rodrigues, E. M. [UNESP]
Cornélio, A. L.G. [UNESP]
Godoi, P. H.
da Costa, P. I. [UNESP]
Rossa-Junior, C. [UNESP]
Faria, G. [UNESP]
Guerreiro Tanomaru, J. M. [UNESP]
Tanomaru-Filho, M. [UNESP]

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Aim: To investigate the biocompatibility, osteogenic bioactivity and mRNA expression of the osteo/odontogenic markers bone morphogenetic protein 2 (BMP-2), osteocalcin (OC) and alkaline phosphatase (ALP), induced by heparin in human dental pulp cells (hDPCs). Methodology: hDPCs were exposed to the heparin, and cell viability was assessed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT), and cell death was evaluated by flow cytometry. Osteogenic bioactivity was evaluated by the alkaline phosphatase (ALP) assay, and the detection of calcium deposits by alizarin red staining (ARS). The gene expression of BMP-2, OC and ALP was quantified with real-time PCR. Statistical analysis was performed with ANOVA and Bonferroni or Tukey post-test and t-test (α = 0.05). Results: Heparin had no cytotoxic effect and did not induce apoptosis. After 3 days, heparin had significantly higher ALP activity in comparison with the control (P < 0.05). Heparin had a significant (P < 0.05) stimulatory effect on the formation of mineralized nodules. BMP-2 and OC mRNA expressions were significantly higher in cells exposed to heparin than control group after 1 day (P < 0.05). Conclusions: Heparin was biocompatible in hDPCs, induced osteogenic bioactivity and enhanced mRNA expression of osteo/odontogenic markers BMP-2 and OC. These results suggest that heparin has potential to induce osteo/odontogenic cell differentiation of hDPCs.



bone morphogenetic protein-2, cytotoxicity, dental pulp cells, heparin, osteogenic bioactivity

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International Endodontic Journal, v. 52, n. 6, p. 829-837, 2019.