Heparin is biocompatible and can induce differentiation of human dental pulp cells

dc.contributor.authorRodrigues, E. M. [UNESP]
dc.contributor.authorCornélio, A. L.G. [UNESP]
dc.contributor.authorGodoi, P. H.
dc.contributor.authorda Costa, P. I. [UNESP]
dc.contributor.authorRossa-Junior, C. [UNESP]
dc.contributor.authorFaria, G. [UNESP]
dc.contributor.authorGuerreiro Tanomaru, J. M. [UNESP]
dc.contributor.authorTanomaru-Filho, M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionQuality and Technology
dc.date.accessioned2019-10-06T15:30:12Z
dc.date.available2019-10-06T15:30:12Z
dc.date.issued2019-06-01
dc.description.abstractAim: To investigate the biocompatibility, osteogenic bioactivity and mRNA expression of the osteo/odontogenic markers bone morphogenetic protein 2 (BMP-2), osteocalcin (OC) and alkaline phosphatase (ALP), induced by heparin in human dental pulp cells (hDPCs). Methodology: hDPCs were exposed to the heparin, and cell viability was assessed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT), and cell death was evaluated by flow cytometry. Osteogenic bioactivity was evaluated by the alkaline phosphatase (ALP) assay, and the detection of calcium deposits by alizarin red staining (ARS). The gene expression of BMP-2, OC and ALP was quantified with real-time PCR. Statistical analysis was performed with ANOVA and Bonferroni or Tukey post-test and t-test (α = 0.05). Results: Heparin had no cytotoxic effect and did not induce apoptosis. After 3 days, heparin had significantly higher ALP activity in comparison with the control (P < 0.05). Heparin had a significant (P < 0.05) stimulatory effect on the formation of mineralized nodules. BMP-2 and OC mRNA expressions were significantly higher in cells exposed to heparin than control group after 1 day (P < 0.05). Conclusions: Heparin was biocompatible in hDPCs, induced osteogenic bioactivity and enhanced mRNA expression of osteo/odontogenic markers BMP-2 and OC. These results suggest that heparin has potential to induce osteo/odontogenic cell differentiation of hDPCs.en
dc.description.affiliationDepartment of Restorative Dentistry Dental School of São Paulo State University-UNESP
dc.description.affiliationNational Institute of Metrology Quality and Technology
dc.description.affiliationDepartment of Bioscience and Biotechnology Applied to Pharmacy of São São Paulo State University-UNESP
dc.description.affiliationDepartment of Diagnosis and Surgery Dental School of São Paulo State University-UNESP
dc.description.affiliationUnespDepartment of Restorative Dentistry Dental School of São Paulo State University-UNESP
dc.description.affiliationUnespDepartment of Bioscience and Biotechnology Applied to Pharmacy of São São Paulo State University-UNESP
dc.description.affiliationUnespDepartment of Diagnosis and Surgery Dental School of São Paulo State University-UNESP
dc.format.extent829-837
dc.identifierhttp://dx.doi.org/10.1111/iej.13061
dc.identifier.citationInternational Endodontic Journal, v. 52, n. 6, p. 829-837, 2019.
dc.identifier.doi10.1111/iej.13061
dc.identifier.issn1365-2591
dc.identifier.issn0143-2885
dc.identifier.scopus2-s2.0-85059638232
dc.identifier.urihttp://hdl.handle.net/11449/187247
dc.language.isoeng
dc.relation.ispartofInternational Endodontic Journal
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectbone morphogenetic protein-2
dc.subjectcytotoxicity
dc.subjectdental pulp cells
dc.subjectheparin
dc.subjectosteogenic bioactivity
dc.titleHeparin is biocompatible and can induce differentiation of human dental pulp cellsen
dc.typeArtigo
unesp.author.lattes7634063102292261[5]
unesp.author.orcid0000-0001-7030-3718[6]
unesp.author.orcid0000-0002-2574-4706[8]
unesp.author.orcid0000-0003-1705-5481[5]

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