Expression of genes related to apoptosis, cell cycle and signaling pathways are independent of TP53 status in urinary bladder cancer cells

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dc.contributor.authorSilva, Glenda N. da UNESP]
dc.contributor.authorEvangelista, Adriane F.
dc.contributor.authorMagalhaes, Danielle A.
dc.contributor.authorMacedo, Claudia
dc.contributor.authorBufalo, Michelle C. [UNESP]
dc.contributor.authorSakamoto-Hojo, Elza T.
dc.contributor.authorPassos, Geraldo A. S.
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:37:36Z
dc.date.available2014-05-20T13:37:36Z
dc.date.issued2011-08-01
dc.description.abstractUrinary bladder cancer is the fourth most common malignancy in the Western world. Transitional cell carcinoma (TCC) is the most common subtype, accounting for about 90% of all bladder cancers. The TP53 gene plays an essential role in the regulation of the cell cycle and apoptosis and therefore contributes to cellular transformation and malignancy; however, little is known about the differential gene expression patterns in human tumors that present with the wild-type or mutated TP53 gene. Therefore, because gene profiling can provide new insights into the molecular biology of bladder cancer, the present study aimed to compare the molecular profiles of bladder cancer cell lines with different TP53 alleles, including the wild type (RT4) and two mutants (5637, with mutations in codons 280 and 72; and T24, a TP53 allele encoding an in-frame deletion of tyrosine 126). Unsupervised hierarchical clustering and gene networks were constructed based on data generated by cDNA microarrays using mRNA from the three cell lines. Differentially expressed genes related to the cell cycle, cell division, cell death, and cell proliferation were observed in the three cell lines. However, the cDNA microarray data did not cluster cell lines based on their TP53 allele. The gene profiles of the RT4 cells were more similar to those of T24 than to those of the 5637 cells. While the deregulation of both the cell cycle and the apoptotic pathways was particularly related to TCC, these alterations were not associated with the TP53 status.en
dc.description.affiliationSão Paulo State Univ, UNESP, Fac Med Botucatu, Botucatu Med Sch, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, UNESP, Biosci Inst, Botucatu, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Biol FFCLRP, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Dent, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Fac Med Botucatu, Botucatu Med Sch, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv São Paulo, UNESP, Biosci Inst, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent4159-4170
dc.identifierhttp://dx.doi.org/10.1007/s11033-010-0536-x
dc.identifier.citationMolecular Biology Reports. Dordrecht: Springer, v. 38, n. 6, p. 4159-4170, 2011.
dc.identifier.doi10.1007/s11033-010-0536-x
dc.identifier.issn0301-4851
dc.identifier.lattes5051118752980903
dc.identifier.urihttp://hdl.handle.net/11449/13036
dc.identifier.wosWOS:000291656800065
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.ispartofjcr1.889
dc.relation.ispartofsjr0,721
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectGene expressionen
dc.subjectGene networken
dc.subjectTP53en
dc.subjectUrinary bladder transitional cell carcinomaen
dc.titleExpression of genes related to apoptosis, cell cycle and signaling pathways are independent of TP53 status in urinary bladder cancer cellsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes5051118752980903
unesp.author.orcid0000-0002-1383-3314[6]
unesp.author.orcid0000-0002-4731-2082[2]
unesp.author.orcid0000-0002-4408-140X[7]
unesp.author.orcid0000-0001-9323-3134[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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