Pheno and genotyping of Staphylococcus aureus, isolated from bovine milk samples from São Paulo State, Brazil

dc.contributor.authorCabral, K. G.
dc.contributor.authorLämmler, C.
dc.contributor.authorZschöck, M.
dc.contributor.authorLangoni, Hélio [UNESP]
dc.contributor.authorDe Sá, M. E. P.
dc.contributor.authorVictória, Cassiano [UNESP]
dc.contributor.authorSilva, A. V. da
dc.contributor.institutionJustus Liebig-Universitat Giessen
dc.contributor.institutionStaatliches Untersuchungsamt Hessen
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:10Z
dc.date.available2014-05-27T11:21:10Z
dc.date.issued2004-11-01
dc.description.abstractIn the present study, 87 Staphylococcus aureus isolates obtained from milk samples of 87 cows with mastitis in 6 different municipal districts of 2 regions of São Paulo State, Brazil, were compared pheno and genotypically. Pulsed-field gel electrophoresis (PFGE) analysis of the strains was performed, and PCR was carried out to detect genes for a number of staphylococcal cell surface proteins, exoproteins, and 3 classes of agr genes. Nine distinct S. aureus lineages (LA-LI) were identified by PFGE. The lineages LA and LE, which accounted together for 63 strains (72.2%), were prevalent and had been collected from all of the 6 municipal districts, indicating a broad geographic distribution of these lineages; LB, LC, LD, LF, LG, LH, and LI, however, were isolated sporadically and accounted for 24 strains (27.8%). Some characteristics, like penicillin resistance and the presence of cap8 and agr class II genes, were associated with the prevalent lineages (LA and LE), and penicillin susceptibility and the presence of cna and cap5 genes were associated with sporadic lineages. According to the present results, some S. aureus lineages possess a combination of genes that confer the propensity to cause and disseminate infection, and only a limited number of clones are responsible for the cases of bovine mastitis on the various farms. © 2004 NRC Canada.en
dc.description.affiliationInst. Tierarztl. Nahrungsmittelkunde Justus Liebig-Universitat Giessen, Ludwigstr. 21, 35390, Gießen
dc.description.affiliationInst. fur Pharmakol. und Toxikologie Justus Liebig-Universitat Giessen, Frankfurter Str. 107, 35392, Gießen
dc.description.affiliationStaatliches Untersuchungsamt Hessen, Marburger Str. 54, 35396, Gießen
dc.description.affiliationDisciplina de Zoonoses Depto. de Higiene Vet. e Saude Pub. Faculdade de Med. Vet. e Zootecnia, Distrito de Rubiao Jr., s/n, 18, 618-000, Botucatu, SP
dc.format.extent901-909
dc.identifierhttp://dx.doi.org/10.1139/w04-082
dc.identifier.citationCanadian Journal of Microbiology, v. 50, n. 11, p. 901-909, 2004.
dc.identifier.doi10.1139/w04-082
dc.identifier.issn0008-4166
dc.identifier.lattes5326072118518067
dc.identifier.scopus2-s2.0-14644425760
dc.identifier.urihttp://hdl.handle.net/11449/67919
dc.language.isoeng
dc.relation.ispartofCanadian Journal of Microbiology
dc.relation.ispartofjcr1.243
dc.relation.ispartofsjr0,579
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectGenotyping
dc.subjectMastitis
dc.subjectPhenotyping
dc.subjectS. aureus
dc.subjectCloning
dc.subjectDrug products
dc.subjectElectrophoresis
dc.subjectGenes
dc.subjectProteins
dc.subjectInfections
dc.subjectPulsed-field gel electrophoresis (PFGE)
dc.subjectStaphylococcus aureus
dc.subjectBacteria
dc.subjectantibiotic agent
dc.subjectcell surface protein
dc.subjectDNA
dc.subjecterythromycin
dc.subjectgentamicin
dc.subjectminocycline
dc.subjectoxacillin
dc.subjectpenicillin G
dc.subjectprotein
dc.subjecttetracycline
dc.subjectmicrobiology
dc.subjectantibiotic sensitivity
dc.subjectbacterial gene
dc.subjectbacterium isolation
dc.subjectBrazil
dc.subjectcell lineage
dc.subjectgene amplification
dc.subjectgenotype
dc.subjectgeographic distribution
dc.subjectmastitis
dc.subjectmilk
dc.subjectnonhuman
dc.subjectpenicillin resistance
dc.subjectphenotype
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectpulsed field gel electrophoresis
dc.subjectAnimals
dc.subjectBacterial Proteins
dc.subjectBacterial Typing Techniques
dc.subjectCattle
dc.subjectDairying
dc.subjectDNA, Bacterial
dc.subjectElectrophoresis, Gel, Pulsed-Field
dc.subjectFemale
dc.subjectGenotype
dc.subjectMastitis, Bovine
dc.subjectMicrobial Sensitivity Tests
dc.subjectMilk
dc.subjectPhenotype
dc.subjectRestriction Mapping
dc.subjectStaphylococcal Infections
dc.subjectBovinae
dc.titlePheno and genotyping of Staphylococcus aureus, isolated from bovine milk samples from São Paulo State, Brazilen
dc.typeArtigo
dcterms.licensehttp://www.nrcresearchpress.com/page/authors/information/rights
unesp.author.lattes5326072118518067
unesp.author.lattes6276174247250429[6]
unesp.author.orcid0000-0002-1957-0324[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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