Myogenic Differentiation Potential of Mesenchymal Stem Cells Derived from Fetal Bovine Bone Marrow

dc.contributor.authorOkamura, Lucas Hidenori [UNESP]
dc.contributor.authorCordero, Paloma
dc.contributor.authorPalomino, Jaime
dc.contributor.authorHugo Parraguez, Victor
dc.contributor.authorGabriel Torres, Cristian
dc.contributor.authorPeralta, Oscar Alejandro
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Chile
dc.contributor.institutionVirginia Tech
dc.date.accessioned2018-11-26T15:46:08Z
dc.date.available2018-11-26T15:46:08Z
dc.date.issued2018-01-01
dc.description.abstractThe myogenic potential of bovine fetal MSC (bfMSC) derived from bone marrow (BM) remains unknown; despite its potential application for the study of myogenesis and its implications for livestock production. In the present study, three protocols for in vitro myogenic differentiation of bfMSC based on the use of DNA methyltransferase inhibitor 5-Aza-2-deoxycytidine (5-Aza), myoblast-secreted factor Galectin-1 (Gal-1), and myoblast culture medium SkGM-2 BulletKit were used. Plastic-adherent bfMSC were isolated from fetal BM collected from abattoir-derived fetuses. Post-thaw viability analyses detected 85.6% bfMSC negative for propidium iodine (PI). Levels of muscle regulatory factors (MRF) MYF5, MYF6, MYOD, and DES mRNA were higher (P<0.05) in bfMSC cultured under 100 mu M of 5-Aza compared to 1 and 10 mu M. Treatment of bfMSC with 10 mu M of 5-Aza resulted in down-regulation of MYOD mRNA (Days 7 to 21) and up-regulation of MYF6 (Day 7), MYF5, and DES mRNA (Day 21). Gal-1 and SkGM-2 BulletKit induced sequential down-regulation of early MRF (MYF5) and up-regulation of intermediate (MYOD) and late MRF (DES) mRNA. Moreover, DES and MYF5 were immunodetected in differentiated bfMSC. In conclusion, protocols evaluated in bfMSC induced progress into myogenic differentiation until certain extent evidenced by changes in MRF gene expression.en
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet, Dept Apoio Prod & Saude Anim, Sao Paulo, Brazil
dc.description.affiliationUniv Chile, Fac Ciencias Vet & Pecuarias, Dept Fomento Prod Anim, Santiago 8820808, Chile
dc.description.affiliationUniv Chile, Fac Ciencias Vet & Pecuarias, Dept Ciencias Biol, Santiago, Chile
dc.description.affiliationUniv Chile, Fac Ciencias Vet & Pecuarias, Dept Ciencias Clin, Santiago, Chile
dc.description.affiliationVirginia Tech, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA USA
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet, Dept Apoio Prod & Saude Anim, Sao Paulo, Brazil
dc.description.sponsorshipOffice of Research and Development, University of Chile
dc.description.sponsorshipgrant Fondequip from the National Commission for Scientific and Technology Research (Conicyt) from the Ministry of Education, Government of Chile
dc.description.sponsorshipIdOffice of Research and Development, University of Chile: 2014-69975
dc.description.sponsorshipIdgrant Fondequip from the National Commission for Scientific and Technology Research (Conicyt) from the Ministry of Education, Government of Chile: EQM120156
dc.format.extent1-11
dc.identifierhttp://dx.doi.org/10.1080/10495398.2016.1276926
dc.identifier.citationAnimal Biotechnology. Philadelphia: Taylor & Francis Inc, v. 29, n. 1, p. 1-11, 2018.
dc.identifier.doi10.1080/10495398.2016.1276926
dc.identifier.fileWOS000419944000001.pdf
dc.identifier.issn1049-5398
dc.identifier.urihttp://hdl.handle.net/11449/160015
dc.identifier.wosWOS:000419944000001
dc.language.isoeng
dc.publisherTaylor & Francis Inc
dc.relation.ispartofAnimal Biotechnology
dc.relation.ispartofsjr0,350
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectBone marrow
dc.subjectcattle
dc.subjectmesenchymal stem cells
dc.subjectmyogenesis
dc.titleMyogenic Differentiation Potential of Mesenchymal Stem Cells Derived from Fetal Bovine Bone Marrowen
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dcterms.rightsHolderTaylor & Francis Inc

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