6-Nitrodopamine potentiates contractions of rat isolated vas deferens induced by noradrenaline, adrenaline, dopamine and electric field stimulation

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dc.contributor.authorBritto-Júnior, José
dc.contributor.authorLima, Antonio Tiago
dc.contributor.authorCampos, Rafael
dc.contributor.authorGião, Alexandre Asturiano
dc.contributor.authorMendes, Gustavo Duarte
dc.contributor.authorFerreira-Novaes Silva, Luiza P.
dc.contributor.authorFregonesi, Adriano
dc.contributor.authorPupo, André Sampaio [UNESP]
dc.contributor.authorAntunes, Edson
dc.contributor.authorDe Nucci, Gilberto
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionSão Leopoldo Mandic (SLMANDIC)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2023-07-29T13:49:12Z
dc.date.available2023-07-29T13:49:12Z
dc.date.issued2023-01-01
dc.description.abstract6-Nitrodopamine (6-ND) is a novel endogenous catecholamine that is released from the rat isolated vas deferens, and has been characterized as a major modulator of the contractility of rat isolated epididymal vas deferens (RIEVD). Drugs such as tricyclic antidepressants, α1 and β1β2 adrenoceptor blockers, act as selective antagonists of the 6-ND receptor in the RIEVD. In the rat isolated atria, 6-ND has a potent positive chronotropic action and causes remarkable potentiation of the positive chronotropic effects induced by dopamine, noradrenaline, and adrenaline. Here, whether 6-ND interacts with the classical catecholamines in the rat isolated vas deferens was investigated. Incubation with 6-ND (0.1 and 1 nM; 30min) caused no contractions in the RIEVD but provoked significant leftward shifts in the concentration-response curves to noradrenaline, adrenaline, and dopamine. Pre-incubation of the RIEVD with 6-ND (1 nM), potentiated the contractions induced by electric-field stimulation (EFS), whereas pre-incubation with 1 nM of dopamine, noradrenaline or adrenaline, did not affect EFS-induced contractions. In tetrodotoxin (1 μM) pre-treated (30 min) RIEVD, pre-incubation with 6-ND (0.1 nM) did not cause leftward shifts in the concentration-dependent contractions induced by noradrenaline, adrenaline, or dopamine. Pre-incubation of the RIEVD with the α2A-adrenoceptor antagonist idazoxan (30 min, 10 nM) did not affect dopamine, noradrenaline, adrenaline, and EFS-induced contractions. However, when idazoxan (10 nM) and 6-ND (0.1 nM) were simultaneously pre-incubated (30 min), a significant potentiation of the EFS-induced contractions of the RIEVD was observed. 6-nitrodopamine causes remarkable potentiation of dopamine, noradrenaline, and adrenaline contractions on the RIEVD, due to activation of adrenergic terminals, possibly via pre-synaptic adrenoceptors.en
dc.description.affiliationDepartment of Pharmacology Faculty of Medical Sciences State University of Campinas (UNICAMP), SP
dc.description.affiliationFaculty of Medicine São Leopoldo Mandic (SLMANDIC), SP
dc.description.affiliationDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.description.affiliationDepartment of Pharmacology Institute of Biomedical Sciences University of São Paulo (USP), SP
dc.description.affiliationUnespDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1007/s00210-023-02478-6
dc.identifier.citationNaunyn-Schmiedeberg's Archives of Pharmacology.
dc.identifier.doi10.1007/s00210-023-02478-6
dc.identifier.issn1432-1912
dc.identifier.issn0028-1298
dc.identifier.scopus2-s2.0-85151549743
dc.identifier.urihttp://hdl.handle.net/11449/248625
dc.language.isoeng
dc.relation.ispartofNaunyn-Schmiedeberg's Archives of Pharmacology
dc.sourceScopus
dc.subjectAtropine
dc.subjectIndomethacin
dc.subjectNitrocatecholamines
dc.subjectReserpine
dc.subjectSynergism
dc.title6-Nitrodopamine potentiates contractions of rat isolated vas deferens induced by noradrenaline, adrenaline, dopamine and electric field stimulationen
dc.typeArtigo

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