Zerumbone Disturbs the Extracellular Matrix of Fluconazole-Resistant Candida albicans Biofilms

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dc.contributor.authorAbreu-Pereira, César Augusto [UNESP]
dc.contributor.authorGorayb-Pereira, Ana Luiza [UNESP]
dc.contributor.authorMenezes Noveletto, João Vinícius [UNESP]
dc.contributor.authorJordão, Cláudia Carolina [UNESP]
dc.contributor.authorPavarina, Ana Cláudia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T16:15:20Z
dc.date.available2023-07-29T16:15:20Z
dc.date.issued2023-05-01
dc.description.abstractThis study assessed the effect of zerumbone (ZER) against fluconazole-resistant (CaR) and -susceptible Candida albicans (CaS) biofilms and verified the influence of ZER on extracellular matrix components. Initially, to determine the treatment conditions, the minimum inhibitory concentration (MIC), the minimum fungicidal concentration (MFC) and the survival curve were evaluated. Biofilms were formed for 48 h and exposed to ZER at concentrations of 128 and 256 µg/mL for 5, 10 and 20 min (n = 12). One group of biofilms did not receive the treatment in order to monitor the effects. The biofilms were evaluated to determine the microbial population (CFU/mL), and the extracellular matrix components (water-soluble polysaccharides (WSP), alkali-soluble polysaccharides (ASPs), proteins and extracellular DNA (eDNA), as well as the biomass (total and insoluble) were quantified. The MIC value of ZER for CaS was 256 μg/mL, and for CaR, it was 64 μg/mL. The survival curve and the MFC value coincided for CaS (256 μg/mL) and CaR (128 μg/mL). ZER reduced the cellular viability by 38.51% for CaS and by 36.99% for CaR. ZER at 256 µg/mL also reduced the total biomass (57%), insoluble biomass (45%), WSP (65%), proteins (18%) and eDNA (78%) of CaS biofilms. In addition, a reduction in insoluble biomass (13%), proteins (18%), WSP (65%), ASP (10%) and eDNA (23%) was also observed in the CaR biofilms. ZER was effective against fluconazole-resistant and -susceptible C. albicans biofilms and disturbed the extracellular matrix.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.3390/jof9050576
dc.identifier.citationJournal of Fungi, v. 9, n. 5, 2023.
dc.identifier.doi10.3390/jof9050576
dc.identifier.issn2309-608X
dc.identifier.scopus2-s2.0-85160512662
dc.identifier.urihttp://hdl.handle.net/11449/250009
dc.language.isoeng
dc.relation.ispartofJournal of Fungi
dc.sourceScopus
dc.subjectbiofilms
dc.subjectCandida albicans
dc.subjectphytotherapy
dc.subjectzerumbone
dc.titleZerumbone Disturbs the Extracellular Matrix of Fluconazole-Resistant Candida albicans Biofilmsen
dc.typeArtigo
unesp.author.orcid0000-0003-0665-9060[1]
unesp.author.orcid0000-0002-9231-1994[5]

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