Genomic and Evolutionary Analysis of Salmonella enterica Serovar Kentucky Sequence Type 198 Isolated From Livestock In East Africa

dc.contributor.authorSaraiva, Mauro de Mesquita Sousa [UNESP]
dc.contributor.authorBenevides, Valdinete Pereira [UNESP]
dc.contributor.authorSilva, Núbia Michelle Vieira da
dc.contributor.authorVarani, Alessandro de Mello [UNESP]
dc.contributor.authorFreitas Neto, Oliveiro Caetano de
dc.contributor.authorBerchieri, Ângelo [UNESP]
dc.contributor.authorDelgado-Suárez, Enrique Jesús
dc.contributor.authorRocha, Alan Douglas de Lima
dc.contributor.authorEguale, Tadesse
dc.contributor.authorMunyalo, Janet Agnes
dc.contributor.authorKariuki, Samuel
dc.contributor.authorGebreyes, Wondwossen Abebe
dc.contributor.authorOliveira, Celso José Bruno de
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionThe Ohio State University
dc.contributor.institutionUniversidade Federal da Paraíba (UFPB)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidad Nacional Autónoma de México (UNAM)
dc.contributor.institutionAddis Ababa University
dc.contributor.institutionKenya Medical Research Institute
dc.date.accessioned2023-03-01T20:55:26Z
dc.date.available2023-03-01T20:55:26Z
dc.date.issued2022-06-20
dc.description.abstractSince its emergence in the beginning of the 90’s, multidrug-resistant (MDR) Salmonella enterica subsp. enterica serovar Kentucky has become a significant public health problem, especially in East Africa. This study aimed to investigate the antimicrobial resistance profile and the genotypic relatedness of Salmonella Kentucky isolated from animal sources in Ethiopia and Kenya (n=19). We also investigated population evolutionary dynamics through phylogenetic and pangenome analyses with additional publicly available Salmonella Kentucky ST198 genomes (n=229). All the 19 sequenced Salmonella Kentucky isolates were identified as ST198. Among these isolates, the predominant genotypic antimicrobial resistance profile observed in ten (59.7%) isolates included the aac(3)-Id, aadA7, strA-strB, blaTEM-1B, sul1, and tet(A) genes, which mediated resistance to gentamicin, streptomycin/spectinomycin, streptomycin, ampicillin, sulfamethoxazole and tetracycline, respectively; and gyrA and parC mutations associated to ciprofloxacin resistance. Four isolates harbored plasmid types Incl1 and/or Col8282; two of them carried both plasmids. Salmonella Pathogenicity islands (SPI-1 to SPI-5) were highly conserved in the 19 sequenced Salmonella Kentucky isolates. Moreover, at least one Pathogenicity Island (SPI 1–4, SPI 9 or C63PI) was identified among the 229 public Salmonella Kentucky genomes. The phylogenetic analysis revealed that almost all Salmonella Kentucky ST198 isolates (17/19) stemmed from a single strain that has accumulated ciprofloxacin resistance-mediating mutations. A total of 8,104 different genes were identified in a heterogenic and still open Salmonella Kentucky ST198 pangenome. Considering the virulence factors and antimicrobial resistance genes detected in Salmonella Kentucky, the implications of this pathogen to public health and the epidemiological drivers for its dissemination must be investigated.en
dc.description.affiliationDepartment of Pathology Theriogenology and One Health Sao Paulo State University (FCAV-Unesp)
dc.description.affiliationDepartment of Preventive Veterinary Medicine College of Veterinary Medicine The Ohio State University
dc.description.affiliationDepartment of Animal Science Center for Agricultural Sciences Federal University of Paraiba (CCA/UFPB)
dc.description.affiliationDepartment of Technology Sao Paulo State University (FCAV-Unesp)
dc.description.affiliationDepartment of Preventive Veterinary Medicine Veterinary School Federal University of Minas Gerais (UFMG)
dc.description.affiliationFacultad de Medicina Veterinaria y Zootecnia Universidad Nacional Autónoma de México (UNAM)
dc.description.affiliationAklilu Lemma Institute of Pathobiology Addis Ababa University
dc.description.affiliationCentre for Microbiology Research Kenya Medical Research Institute
dc.description.affiliationGlobal One Health Initiative (GOHi) The Ohio State University
dc.description.affiliationUnespDepartment of Pathology Theriogenology and One Health Sao Paulo State University (FCAV-Unesp)
dc.description.affiliationUnespDepartment of Technology Sao Paulo State University (FCAV-Unesp)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFinanciadora de Estudos e Projetos
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCAPES: 88881.131934/2016-01
dc.description.sponsorshipIdCAPES: 88881.311776/2018-01
dc.description.sponsorshipIdCAPES: Finance Code 001
dc.identifierhttp://dx.doi.org/10.3389/fcimb.2022.772829
dc.identifier.citationFrontiers in Cellular and Infection Microbiology, v. 12.
dc.identifier.doi10.3389/fcimb.2022.772829
dc.identifier.issn2235-2988
dc.identifier.scopus2-s2.0-85133613488
dc.identifier.urihttp://hdl.handle.net/11449/241290
dc.language.isoeng
dc.relation.ispartofFrontiers in Cellular and Infection Microbiology
dc.sourceScopus
dc.subjectantimicrobial resistance
dc.subjectfoodborne pathogen
dc.subjectgenetic diversity
dc.subjectSalmonella pathogenicity islands
dc.subjectsalmonellosis
dc.subjectwhole genome sequencing
dc.titleGenomic and Evolutionary Analysis of Salmonella enterica Serovar Kentucky Sequence Type 198 Isolated From Livestock In East Africaen
dc.typeArtigo
unesp.departmentPatologia Veterinária - FCAVpt
unesp.departmentTecnologia - FCAVpt

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