Genomic and Evolutionary Analysis of Salmonella enterica Serovar Kentucky Sequence Type 198 Isolated From Livestock In East Africa
dc.contributor.author | Saraiva, Mauro de Mesquita Sousa [UNESP] | |
dc.contributor.author | Benevides, Valdinete Pereira [UNESP] | |
dc.contributor.author | Silva, Núbia Michelle Vieira da | |
dc.contributor.author | Varani, Alessandro de Mello [UNESP] | |
dc.contributor.author | Freitas Neto, Oliveiro Caetano de | |
dc.contributor.author | Berchieri, Ângelo [UNESP] | |
dc.contributor.author | Delgado-Suárez, Enrique Jesús | |
dc.contributor.author | Rocha, Alan Douglas de Lima | |
dc.contributor.author | Eguale, Tadesse | |
dc.contributor.author | Munyalo, Janet Agnes | |
dc.contributor.author | Kariuki, Samuel | |
dc.contributor.author | Gebreyes, Wondwossen Abebe | |
dc.contributor.author | Oliveira, Celso José Bruno de | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | The Ohio State University | |
dc.contributor.institution | Universidade Federal da Paraíba (UFPB) | |
dc.contributor.institution | Universidade Federal de Minas Gerais (UFMG) | |
dc.contributor.institution | Universidad Nacional Autónoma de México (UNAM) | |
dc.contributor.institution | Addis Ababa University | |
dc.contributor.institution | Kenya Medical Research Institute | |
dc.date.accessioned | 2023-03-01T20:55:26Z | |
dc.date.available | 2023-03-01T20:55:26Z | |
dc.date.issued | 2022-06-20 | |
dc.description.abstract | Since its emergence in the beginning of the 90’s, multidrug-resistant (MDR) Salmonella enterica subsp. enterica serovar Kentucky has become a significant public health problem, especially in East Africa. This study aimed to investigate the antimicrobial resistance profile and the genotypic relatedness of Salmonella Kentucky isolated from animal sources in Ethiopia and Kenya (n=19). We also investigated population evolutionary dynamics through phylogenetic and pangenome analyses with additional publicly available Salmonella Kentucky ST198 genomes (n=229). All the 19 sequenced Salmonella Kentucky isolates were identified as ST198. Among these isolates, the predominant genotypic antimicrobial resistance profile observed in ten (59.7%) isolates included the aac(3)-Id, aadA7, strA-strB, blaTEM-1B, sul1, and tet(A) genes, which mediated resistance to gentamicin, streptomycin/spectinomycin, streptomycin, ampicillin, sulfamethoxazole and tetracycline, respectively; and gyrA and parC mutations associated to ciprofloxacin resistance. Four isolates harbored plasmid types Incl1 and/or Col8282; two of them carried both plasmids. Salmonella Pathogenicity islands (SPI-1 to SPI-5) were highly conserved in the 19 sequenced Salmonella Kentucky isolates. Moreover, at least one Pathogenicity Island (SPI 1–4, SPI 9 or C63PI) was identified among the 229 public Salmonella Kentucky genomes. The phylogenetic analysis revealed that almost all Salmonella Kentucky ST198 isolates (17/19) stemmed from a single strain that has accumulated ciprofloxacin resistance-mediating mutations. A total of 8,104 different genes were identified in a heterogenic and still open Salmonella Kentucky ST198 pangenome. Considering the virulence factors and antimicrobial resistance genes detected in Salmonella Kentucky, the implications of this pathogen to public health and the epidemiological drivers for its dissemination must be investigated. | en |
dc.description.affiliation | Department of Pathology Theriogenology and One Health Sao Paulo State University (FCAV-Unesp) | |
dc.description.affiliation | Department of Preventive Veterinary Medicine College of Veterinary Medicine The Ohio State University | |
dc.description.affiliation | Department of Animal Science Center for Agricultural Sciences Federal University of Paraiba (CCA/UFPB) | |
dc.description.affiliation | Department of Technology Sao Paulo State University (FCAV-Unesp) | |
dc.description.affiliation | Department of Preventive Veterinary Medicine Veterinary School Federal University of Minas Gerais (UFMG) | |
dc.description.affiliation | Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Autónoma de México (UNAM) | |
dc.description.affiliation | Aklilu Lemma Institute of Pathobiology Addis Ababa University | |
dc.description.affiliation | Centre for Microbiology Research Kenya Medical Research Institute | |
dc.description.affiliation | Global One Health Initiative (GOHi) The Ohio State University | |
dc.description.affiliationUnesp | Department of Pathology Theriogenology and One Health Sao Paulo State University (FCAV-Unesp) | |
dc.description.affiliationUnesp | Department of Technology Sao Paulo State University (FCAV-Unesp) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Financiadora de Estudos e Projetos | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorshipId | CAPES: 88881.131934/2016-01 | |
dc.description.sponsorshipId | CAPES: 88881.311776/2018-01 | |
dc.description.sponsorshipId | CAPES: Finance Code 001 | |
dc.identifier | http://dx.doi.org/10.3389/fcimb.2022.772829 | |
dc.identifier.citation | Frontiers in Cellular and Infection Microbiology, v. 12. | |
dc.identifier.doi | 10.3389/fcimb.2022.772829 | |
dc.identifier.issn | 2235-2988 | |
dc.identifier.scopus | 2-s2.0-85133613488 | |
dc.identifier.uri | http://hdl.handle.net/11449/241290 | |
dc.language.iso | eng | |
dc.relation.ispartof | Frontiers in Cellular and Infection Microbiology | |
dc.source | Scopus | |
dc.subject | antimicrobial resistance | |
dc.subject | foodborne pathogen | |
dc.subject | genetic diversity | |
dc.subject | Salmonella pathogenicity islands | |
dc.subject | salmonellosis | |
dc.subject | whole genome sequencing | |
dc.title | Genomic and Evolutionary Analysis of Salmonella enterica Serovar Kentucky Sequence Type 198 Isolated From Livestock In East Africa | en |
dc.type | Artigo | |
unesp.department | Patologia Veterinária - FCAV | pt |
unesp.department | Tecnologia - FCAV | pt |