Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)

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dc.contributor.authorNetto, D. P.
dc.contributor.authorChiacchio, Simone Biagio [UNESP]
dc.contributor.authorBicudo, P. L. [UNESP]
dc.contributor.authorAlfieri, A. A.
dc.contributor.authorBalarim, M. R. S.
dc.contributor.authorNascimento, N.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:39:22Z
dc.date.available2014-05-20T13:39:22Z
dc.date.issued2004-01-01
dc.description.abstractNatural (NV) and Cobalto60-irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0) and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV) and the other natural venom (NV). Three antigen doses (venom) were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (CBC), total plasma protein, fibrinogen, albumin, and globulin. At the end of the experiment, the animals were challenged with a LD50 for sheep and showed no signs of envenoming. The two groups did not present clinical alterations. Results of the total leukocyte count did not present interaction or time factor effect for both groups, but there was a different action between them, with the NV group presenting more cells than the IrV group. The leukocyte increase to 13,000/ml indicates that slight leukocytosis occurred in the week after the first inoculation in the NV group. There was no statistically significant difference between groups in the absolute count of segmented neutrophils, eosinophils, and lymphocytes but there were statistically significant oscillations in values at the different collecting times. The NV group presented an increase in the absolute neutrophil count after the first inoculation that persisted for 5 weeks. In the IrV group, the increase in neutrophils occurred only in the first week returning to normal in the following weeks. The alterations in the neutrophil count are indicative of systemic inflammatory response related to cytokine release; response was more marked in the NV group, showing its greater toxicity.en
dc.description.affiliationUniversidade Estadual de Londrina (UEL) Faculdade de Medicina Veterinária e Zootecnia Departamento de Medicina Veterinária Preventiva
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia
dc.description.affiliationUniversidade de São Paulo Instituto de Pesquisas Energéticas e Nucleares
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia
dc.format.extent34-52
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992004000100004
dc.identifier.citationJournal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 10, n. 1, p. 34-52, 2004.
dc.identifier.doi10.1590/S1678-91992004000100004
dc.identifier.fileS1678-91992004000100004.pdf
dc.identifier.issn1678-9199
dc.identifier.lattes3353201621529431
dc.identifier.scieloS1678-91992004000100004
dc.identifier.urihttp://hdl.handle.net/11449/13661
dc.language.isoeng
dc.publisherUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjecthematologyen
dc.subjectgamma radiationen
dc.subjectirradiated crotalic venomen
dc.subjectcrotalic antiserumen
dc.subjectovineen
dc.subjectCrotalus durissus terrificusen
dc.titleHematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)en
dc.typeArtigo
unesp.author.lattes3353201621529431
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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