Characterization of ruminal bacteria in grazing nellore steers

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dc.contributor.authorde Jesus, Raphael B. [UNESP]
dc.contributor.authorGranja-Salcedo, Yury T. [UNESP]
dc.contributor.authorMessana, Juliana D. [UNESP]
dc.contributor.authorKishi, Luciano T. [UNESP]
dc.contributor.authorLemos, Eliana G. M. [UNESP]
dc.contributor.authorde Souza, Jackson Antonio M. [UNESP]
dc.contributor.authorBerchielli, Telma T. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T01:44:59Z
dc.date.available2020-12-12T01:44:59Z
dc.date.issued2019-10-01
dc.description.abstractBackground: Rumen microorganisms have developed a series of complex interactions, representing one of the best examples of symbiosis between microorganisms in nature. Conventional taxonomic methods based on culture techniques are being replaced by molecular techniques that are faster and more accurate. Objective: To characterize rumen bacterial diversity of Nellore steers grazing on tropical pastures by sequencing the 16S rRNA gene using Illumina sequencing. Methods: Three rumen-cannulated Nellore steers were used. The liquid and solid fractions of the rumen contents were processed to extract metagenomic DNA, and the V1 and V2 hypervariable regions of the 16S rRNA gene were sequenced using Illumina sequencing. Results: A total of 11,407,000 reads of adequate quality were generated, and 812 operational taxonomic units (OTUs) were found at the species level. Twenty-seven phyla were identified, and the predominant phyla were Firmicutes (23%), Bacteroidetes (14%), Proteobacteria (10%), Spirochaetes (9%), Fibrobacteres (7%), Tenericutes (5%), and Actinobacteria (2%), which represented 70% of the total phyla identified in the rumen content. Conclusion: Rumen environment in grazing Nellore steers showed high bacterial diversity, with Firmicutes, Bacteroidetes, Proteobacteria, Spirochaetes, and Fibrobacteres as the predominant phyla.en
dc.description.affiliationDepartamento de Zootecnia Faculdade de Ciências Agrarias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP), Câmpus Jaboticabal
dc.description.affiliationDepartamento de Tecnologia Faculdade de Ciências Agrarias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP), Câmpus Jaboticabal
dc.description.affiliationDepartamento de Biologia Faculdade de Ciências Agrarias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP), Câmpus Jaboticabal
dc.description.affiliationUnespDepartamento de Zootecnia Faculdade de Ciências Agrarias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP), Câmpus Jaboticabal
dc.description.affiliationUnespDepartamento de Tecnologia Faculdade de Ciências Agrarias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP), Câmpus Jaboticabal
dc.description.affiliationUnespDepartamento de Biologia Faculdade de Ciências Agrarias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP), Câmpus Jaboticabal
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipUniversidade Estadual Paulista
dc.description.sponsorshipIdFAPESP: 2013/ 23851-6
dc.description.sponsorshipIdFAPESP: 2015/17966-0
dc.description.sponsorshipIdCNPq: 475844/2012-2
dc.format.extent248-260
dc.identifierhttp://dx.doi.org/10.17533/udea.rccp.v32n4a01
dc.identifier.citationRevista Colombiana de Ciencias Pecuarias, v. 32, n. 4, p. 248-260, 2019.
dc.identifier.doi10.17533/udea.rccp.v32n4a01
dc.identifier.issn2256-2958
dc.identifier.issn0120-0690
dc.identifier.scopus2-s2.0-85074661927
dc.identifier.urihttp://hdl.handle.net/11449/199626
dc.language.isoeng
dc.relation.ispartofRevista Colombiana de Ciencias Pecuarias
dc.sourceScopus
dc.subjectBacterial diversity
dc.subjectCynodon spp
dc.subjectFirmicutes
dc.subjectNellore
dc.subjectNew generation sequencing
dc.subjectRumen microorganisms
dc.subjectRuminal bacteria
dc.subjectRuminant
dc.subjectSteers
dc.subjectZebu
dc.titleCharacterization of ruminal bacteria in grazing nellore steersen
dc.titleCaracterización de bacterias ruminales en novillos nelore en pastoreoes
dc.titleCaraterização bacteriana ruminal em novilhos nelore em pastejopt
dc.typeArtigo

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