cry1 genes from Bacillus thuringiensis: specificity determination and implications for primer design
Nenhuma Miniatura disponível
Data
2009-12-01
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Springer
Tipo
Artigo
Direito de acesso
Acesso restrito
Resumo
Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic sequences from five cry1 subclasses was carried out and characterized by molecular tools. The design of new primers allows for more precise selection of B. thuringiensis isolates, helping to better direct the programs employing biological control.
Descrição
Palavras-chave
Idioma
Inglês
Como citar
Biotechnology Letters. Dordrecht: Springer, v. 31, n. 12, p. 1891-1897, 2009.