cry1 genes from Bacillus thuringiensis: specificity determination and implications for primer design

Nenhuma Miniatura disponível

Data

2009-12-01

Título da Revista

ISSN da Revista

Título de Volume

Editor

Springer

Resumo

Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic sequences from five cry1 subclasses was carried out and characterized by molecular tools. The design of new primers allows for more precise selection of B. thuringiensis isolates, helping to better direct the programs employing biological control.

Descrição

Palavras-chave

Cry proteins, cry1 subclasses, DNA sequencing, DNA homology, PCR

Como citar

Biotechnology Letters. Dordrecht: Springer, v. 31, n. 12, p. 1891-1897, 2009.

Coleções