cry1 genes from Bacillus thuringiensis: specificity determination and implications for primer design

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dc.contributor.authorDavolos, Camila C. [UNESP]
dc.contributor.authorGuidelli-Thuler, Ana M. [UNESP]
dc.contributor.authorde Abreu, Irlan L. [UNESP]
dc.contributor.authorSena, Janete A.D. [UNESP]
dc.contributor.authorLemos, Manoel V F
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:30:32Z
dc.date.available2014-05-20T15:30:32Z
dc.date.issued2009-12-01
dc.description.abstractSome pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic sequences from five cry1 subclasses was carried out and characterized by molecular tools. The design of new primers allows for more precise selection of B. thuringiensis isolates, helping to better direct the programs employing biological control.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, Lab Genet Bacterias & Biotecnol Aplicada, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, Lab Genet Bacterias & Biotecnol Aplicada, São Paulo, Brazil
dc.format.extent1891-1897
dc.identifierhttp://dx.doi.org/10.1007/s10529-009-0088-0
dc.identifier.citationBiotechnology Letters. Dordrecht: Springer, v. 31, n. 12, p. 1891-1897, 2009.
dc.identifier.doi10.1007/s10529-009-0088-0
dc.identifier.issn0141-5492
dc.identifier.urihttp://hdl.handle.net/11449/39888
dc.identifier.wosWOS:000271481200012
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofBiotechnology Letters
dc.relation.ispartofjcr1.846
dc.relation.ispartofsjr0,621
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCry proteinsen
dc.subjectcry1 subclassesen
dc.subjectDNA sequencingen
dc.subjectDNA homologyen
dc.subjectPCRen
dc.titlecry1 genes from Bacillus thuringiensis: specificity determination and implications for primer designen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer

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