cry1 genes from Bacillus thuringiensis: specificity determination and implications for primer design
dc.contributor.author | Davolos, Camila C. [UNESP] | |
dc.contributor.author | Guidelli-Thuler, Ana M. [UNESP] | |
dc.contributor.author | de Abreu, Irlan L. [UNESP] | |
dc.contributor.author | Sena, Janete A.D. [UNESP] | |
dc.contributor.author | Lemos, Manoel V F | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-20T15:30:32Z | |
dc.date.available | 2014-05-20T15:30:32Z | |
dc.date.issued | 2009-12-01 | |
dc.description.abstract | Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic sequences from five cry1 subclasses was carried out and characterized by molecular tools. The design of new primers allows for more precise selection of B. thuringiensis isolates, helping to better direct the programs employing biological control. | en |
dc.description.affiliation | Univ Estadual Paulista, Fac Ciencias Agr & Vet, Lab Genet Bacterias & Biotecnol Aplicada, São Paulo, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Fac Ciencias Agr & Vet, Lab Genet Bacterias & Biotecnol Aplicada, São Paulo, Brazil | |
dc.format.extent | 1891-1897 | |
dc.identifier | http://dx.doi.org/10.1007/s10529-009-0088-0 | |
dc.identifier.citation | Biotechnology Letters. Dordrecht: Springer, v. 31, n. 12, p. 1891-1897, 2009. | |
dc.identifier.doi | 10.1007/s10529-009-0088-0 | |
dc.identifier.issn | 0141-5492 | |
dc.identifier.uri | http://hdl.handle.net/11449/39888 | |
dc.identifier.wos | WOS:000271481200012 | |
dc.language.iso | eng | |
dc.publisher | Springer | |
dc.relation.ispartof | Biotechnology Letters | |
dc.relation.ispartofjcr | 1.846 | |
dc.relation.ispartofsjr | 0,621 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | Cry proteins | en |
dc.subject | cry1 subclasses | en |
dc.subject | DNA sequencing | en |
dc.subject | DNA homology | en |
dc.subject | PCR | en |
dc.title | cry1 genes from Bacillus thuringiensis: specificity determination and implications for primer design | en |
dc.type | Artigo | |
dcterms.license | http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 | |
dcterms.rightsHolder | Springer |
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