Improved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile

dc.contributor.authorLeao, B. C. S. [UNESP]
dc.contributor.authorRocha-Frigoni, N. A. S. [UNESP]
dc.contributor.authorCabral, E. C.
dc.contributor.authorCoelho, M. B.
dc.contributor.authorFerreira, C. R.
dc.contributor.authorEberlin, M. N.
dc.contributor.authorAccorsi, M. F. [UNESP]
dc.contributor.authorNogueira, E.
dc.contributor.authorMingoti, G. Z. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2015-10-21T13:10:08Z
dc.date.available2015-10-21T13:10:08Z
dc.date.issued2015-07-01
dc.description.abstractThe aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12-conjugated isomers of linoleic acid (CIA) during different steps of the in vitro production (IVM, IVC, or IVM IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CIA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos'lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%-84.8%) and blastocyst development (44.8%-51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CIA was added to the IVM medium (82.6%) or to the IVM + IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts'lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CIA on the embryonic PC and SM profiles were observed when the supplement was added to IVM + IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CIA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CIA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is dependent on the treatment length. Monitoring the lipid profile of embryonic membranes may improve the CIA supplementation strategy and facilitate the development of new IVC systems to improve the cryopreservation of bovine embryos and those of other domestic species.en
dc.description.affiliationUniversidade Estadual Paulista (UNESP) - School of Veterinary Medicine, Department of Animal Health, Laboratory of Physiology of Reproduction, Araçatuba, São Paulo, Brazil
dc.description.affiliationUniversity of Campinas (UNICAMP) - ThoMSon Mass Spectrometry Laboratory, Chemistry Institute, Campinas, São Paulo, Brazil
dc.description.affiliationBrazilian Agricultural Research Corporation, EMBRAPA Pantanal, Corumbá, Mato Grosso do Sul, Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) - School of Veterinary Medicine, Department of Animal Health, Laboratory of Physiology of Reproduction, Araçatuba, São Paulo, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent127-136
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0093691X15001272
dc.identifier.citationTheriogenology. New York: Elsevier Science Inc, v. 84, n. 1, p. 127-136, 2015.
dc.identifier.doi10.1016/j.theriogenology.2015.02.023
dc.identifier.issn0093-691X
dc.identifier.orcid0000-0002-3059-4458
dc.identifier.urihttp://hdl.handle.net/11449/128469
dc.identifier.wosWOS:000355572900017
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBovine embryoen
dc.subjectConjugated linoleic aciden
dc.subjectIn vitro productionen
dc.subjectMass spectrometryen
dc.subjectLipid profileen
dc.subjectCryosurvivalen
dc.titleImproved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profileen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-3059-4458[9]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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