Effect of Photobiomodulation Therapy Associated With Biphasic Phosphate Calcium on Bone Repair: A Histomorphometric Study in Rats

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dc.contributor.authorDe Marco, Andréa Carvalho [UNESP]
dc.contributor.authorTorquato, Letícia Cavassini [UNESP]
dc.contributor.authorRibeiro, Tatiana Camacho
dc.contributor.authorNunes, Camilla Moretto
dc.contributor.authorBernardo, Daniella Vicensotto
dc.contributor.authorMaciel, Clarissa Carvalho Martins [UNESP]
dc.contributor.authorPereira, Kauê Alberto [UNESP]
dc.contributor.authorJardini, Maria Aparecida Neves [UNESP]
dc.contributor.authorSantamaria, Mauro Pedrine [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionFaculdade Santo Antônio
dc.date.accessioned2023-07-29T16:05:15Z
dc.date.available2023-07-29T16:05:15Z
dc.date.issued2022-01-01
dc.description.abstractIntroduction: This study aimed to investigate the effects of photobiomodulation (PBM) therapy associated with biphasic calcium phosphate on calvaria critical defects in rats. Methods: Forty-eight (90 days old) adult male rats (Rattus norvegicus, Albinus variation, Wistar) received critical defects of 5 mm in diameter, which were made on their skull, and they were randomly assigned into the following groups: C-blood clot, B-biphasic calcium phosphate, L-photobiomodulation therapy, and B + L-biphasic calcium phosphate + photobiomodulation therapy. A low-level a gallium aluminum arsenide (GaAlAs) laser was applied in a single dose during surgery, in a wavelength of 660 nm and total energy density of 45 J/cm2. On 30th and 60th days, the animals from each group were euthanized. Histological and histomorphometric analyses were performed. Results: In 30 days, almost all specimens (C, L, B and B + L) showed bone neoformation areas in regions near the borders of the surgical defect. In 60 days, in many specimens (C, L, B, B + L), it was possible to see a narrow neoformed bone structure along almost the whole extension of the surgical defect, though it was thinner than the original calvary bone. Data were recorded as mean ± standard deviation, and after normality was tested, a suitable statistical test was applied (α = 5%). On day 60, there was a statistically significant difference when comparing the proportion of neoformation area between group L (0.52% ± 0.13) and group B + L (0.20% ± 0.08). Group L showed a difference compared with all the groups when we compared the remaining distance between the edges of neoformed bone (C × L, P = 0.0431; B × L, P = 0.0386; L × B + L, P = 0.0352), demonstrating a great defect closure. Conclusion: Our findings suggest that although biphasic calcium phosphate exerts some osteogenic activity during bone repair, PBM therapy is not able to modulate this processen
dc.description.affiliationDepartment of Diagnosis and Surgery – Division of Periodontology Sao Paulo State University (UNESP) Institute of Science and Technology
dc.description.affiliationDivision of Periodontology Pre-clinical Faculdade Santo Antônio
dc.description.affiliationUnespDepartment of Diagnosis and Surgery – Division of Periodontology Sao Paulo State University (UNESP) Institute of Science and Technology
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/09614-7
dc.identifierhttp://dx.doi.org/10.34172/jlms.2022.33
dc.identifier.citationJournal of Lasers in Medical Sciences, v. 13.
dc.identifier.doi10.34172/jlms.2022.33
dc.identifier.issn2228-6721
dc.identifier.issn2008-9783
dc.identifier.scopus2-s2.0-85147766038
dc.identifier.urihttp://hdl.handle.net/11449/249643
dc.language.isoeng
dc.relation.ispartofJournal of Lasers in Medical Sciences
dc.sourceScopus
dc.subjectBiocompatible materials
dc.subjectBone regeneration
dc.subjectBone substitutes
dc.subjectLaser therapy
dc.subjectRats
dc.titleEffect of Photobiomodulation Therapy Associated With Biphasic Phosphate Calcium on Bone Repair: A Histomorphometric Study in Ratsen
dc.typeArtigo

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