Effect of Photobiomodulation Therapy Associated With Biphasic Phosphate Calcium on Bone Repair: A Histomorphometric Study in Rats
dc.contributor.author | De Marco, Andréa Carvalho [UNESP] | |
dc.contributor.author | Torquato, Letícia Cavassini [UNESP] | |
dc.contributor.author | Ribeiro, Tatiana Camacho | |
dc.contributor.author | Nunes, Camilla Moretto | |
dc.contributor.author | Bernardo, Daniella Vicensotto | |
dc.contributor.author | Maciel, Clarissa Carvalho Martins [UNESP] | |
dc.contributor.author | Pereira, Kauê Alberto [UNESP] | |
dc.contributor.author | Jardini, Maria Aparecida Neves [UNESP] | |
dc.contributor.author | Santamaria, Mauro Pedrine [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | Faculdade Santo Antônio | |
dc.date.accessioned | 2023-07-29T16:05:15Z | |
dc.date.available | 2023-07-29T16:05:15Z | |
dc.date.issued | 2022-01-01 | |
dc.description.abstract | Introduction: This study aimed to investigate the effects of photobiomodulation (PBM) therapy associated with biphasic calcium phosphate on calvaria critical defects in rats. Methods: Forty-eight (90 days old) adult male rats (Rattus norvegicus, Albinus variation, Wistar) received critical defects of 5 mm in diameter, which were made on their skull, and they were randomly assigned into the following groups: C-blood clot, B-biphasic calcium phosphate, L-photobiomodulation therapy, and B + L-biphasic calcium phosphate + photobiomodulation therapy. A low-level a gallium aluminum arsenide (GaAlAs) laser was applied in a single dose during surgery, in a wavelength of 660 nm and total energy density of 45 J/cm2. On 30th and 60th days, the animals from each group were euthanized. Histological and histomorphometric analyses were performed. Results: In 30 days, almost all specimens (C, L, B and B + L) showed bone neoformation areas in regions near the borders of the surgical defect. In 60 days, in many specimens (C, L, B, B + L), it was possible to see a narrow neoformed bone structure along almost the whole extension of the surgical defect, though it was thinner than the original calvary bone. Data were recorded as mean ± standard deviation, and after normality was tested, a suitable statistical test was applied (α = 5%). On day 60, there was a statistically significant difference when comparing the proportion of neoformation area between group L (0.52% ± 0.13) and group B + L (0.20% ± 0.08). Group L showed a difference compared with all the groups when we compared the remaining distance between the edges of neoformed bone (C × L, P = 0.0431; B × L, P = 0.0386; L × B + L, P = 0.0352), demonstrating a great defect closure. Conclusion: Our findings suggest that although biphasic calcium phosphate exerts some osteogenic activity during bone repair, PBM therapy is not able to modulate this process | en |
dc.description.affiliation | Department of Diagnosis and Surgery – Division of Periodontology Sao Paulo State University (UNESP) Institute of Science and Technology | |
dc.description.affiliation | Division of Periodontology Pre-clinical Faculdade Santo Antônio | |
dc.description.affiliationUnesp | Department of Diagnosis and Surgery – Division of Periodontology Sao Paulo State University (UNESP) Institute of Science and Technology | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorshipId | FAPESP: 2015/09614-7 | |
dc.identifier | http://dx.doi.org/10.34172/jlms.2022.33 | |
dc.identifier.citation | Journal of Lasers in Medical Sciences, v. 13. | |
dc.identifier.doi | 10.34172/jlms.2022.33 | |
dc.identifier.issn | 2228-6721 | |
dc.identifier.issn | 2008-9783 | |
dc.identifier.scopus | 2-s2.0-85147766038 | |
dc.identifier.uri | http://hdl.handle.net/11449/249643 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Lasers in Medical Sciences | |
dc.source | Scopus | |
dc.subject | Biocompatible materials | |
dc.subject | Bone regeneration | |
dc.subject | Bone substitutes | |
dc.subject | Laser therapy | |
dc.subject | Rats | |
dc.title | Effect of Photobiomodulation Therapy Associated With Biphasic Phosphate Calcium on Bone Repair: A Histomorphometric Study in Rats | en |
dc.type | Artigo |