Measurement of thrombopoietic activity through the quantification of megakaryocytes in bone marrow cytology and reticulated platelets

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dc.contributor.authorSilva, L. F. N. [UNESP]
dc.contributor.authorGolim, M. A. [UNESP]
dc.contributor.authorTakahira, Regina Kiomi [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:39:39Z
dc.date.available2014-05-20T13:39:39Z
dc.date.issued2012-08-01
dc.description.abstractReticulated platelets are considered as marker for bone marrow thrombopoiesis. The aim of the study was evaluate the role of reticulated platelets as markers of thrombopoiesis in dogs. Reticulated platelets analysis by flow cytometry and megakaryocyte quantification by bone marrow cytology were determined ill 29 healthy adult dogs (control group), 14 dogs with thrombocytopenia without megakaryocytic hypoplasia (group A) and 14 dogs with thrombocytopenia which presented megakaryocytic hypoplasia (group B), detected by bone marrow aspiration cytology. Blood samples were collected and the platelet rich plasma was obtained for reticulated platelets quantification in flow cytometry. Megakaryocytes were quantified in aspiration cytology by two techniques in marrow particles, and correlated to reticulated platelets counts. There are no differences between megakaryocyte quantification. Although there is no correlation between reticulated platelet values and megakaryocyte in bone marrow cytology, the interpretation of reticulated platelet values can be based both on absolute or relative corrected values. (C) 2011 Elsevier Ltd. All rights reserved.en
dc.description.affiliationSão Paulo State Univ Unesp, Dept Vet Clin, Sch Vet Med & Anim Sci, Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ Unesp, Lab Flow Cytometry, Blood Bank, Sch Med, Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ Unesp, Dept Vet Clin, Sch Vet Med & Anim Sci, Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ Unesp, Lab Flow Cytometry, Blood Bank, Sch Med, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent313-317
dc.identifierhttp://dx.doi.org/10.1016/j.rvsc.2011.07.005
dc.identifier.citationResearch In Veterinary Science. Oxford: Elsevier B.V., v. 93, n. 1, p. 313-317, 2012.
dc.identifier.doi10.1016/j.rvsc.2011.07.005
dc.identifier.issn0034-5288
dc.identifier.lattes3517825410178813
dc.identifier.orcid0000-0003-3323-4199
dc.identifier.urihttp://hdl.handle.net/11449/13760
dc.identifier.wosWOS:000305852200058
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofResearch in Veterinary Science
dc.relation.ispartofjcr1.616
dc.relation.ispartofsjr0,593
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectReticulated plateletsen
dc.subjectMegakaryocytesen
dc.subjectDogen
dc.subjectBone marrowen
dc.subjectFlow cytometryen
dc.titleMeasurement of thrombopoietic activity through the quantification of megakaryocytes in bone marrow cytology and reticulated plateletsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes3517825410178813
unesp.author.orcid0000-0003-3323-4199[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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