Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manner

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dc.contributor.authorCavariani, Marilia Martins [UNESP]
dc.contributor.authorde Mello Santos, Talita [UNESP]
dc.contributor.authorChuffa, Luiz Gustavo de Almeida [UNESP]
dc.contributor.authorPinheiro, Patrícia Fernanda Felipe [UNESP]
dc.contributor.authorScarano, Wellerson Rodrigo [UNESP]
dc.contributor.authorDomeniconi, Raquel Fantin [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-03-02T00:28:25Z
dc.date.available2023-03-02T00:28:25Z
dc.date.issued2022-04-19
dc.description.abstractNutrition is an environmental factor able to activate physiological interactions between fetus and mother. Maternal protein restriction is able to alter sperm parameters associated with epididymal functions. Since correct development and functioning of the epididymides are fundamental for mammalian reproductive success, this study investigated the effects of maternal protein restriction on epididymal morphology and morphometry in rat offspring as well as on the expression of Src, Cldn-1, AR, ER, aromatase p450, and 5α-reductase in different stages of postnatal epididymal development. For this purpose, pregnant females were allocated to normal-protein (NP—17% protein) and low-protein (LP—6% protein) groups that received specific diets during gestation and lactation. After weaning, male offspring was provided only normal-protein diet until the ages of 21, 44, and 120 days, when they were euthanized and their epididymides collected. Maternal protein restriction decreased genital organs weight as well as crown-rump length and anogenital distance at all ages. Although the low-protein diet did not change the integrity of the epididymal epithelium, we observed decreases in tubular diameter, epithelial height and luminal diameter of the epididymal duct in 21-day-old LP animals. The maternal low-protein diet changed AR, ERα, ERβ, Src 416, and Src 527 expression in offspring epididymides in an age-dependent manner. Finally, maternal protein restriction increased Cldn-1 expression throughout the epididymides at all analyzed ages. Although some of these changes did not remain until adulthood, the insufficient supply of proteins in early life altered the structure and functioning of the epididymis in important periods of postnatal development.en
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.identifierhttp://dx.doi.org/10.3389/fcell.2022.816637
dc.identifier.citationFrontiers in Cell and Developmental Biology, v. 10.
dc.identifier.doi10.3389/fcell.2022.816637
dc.identifier.issn2296-634X
dc.identifier.scopus2-s2.0-85129511539
dc.identifier.urihttp://hdl.handle.net/11449/241802
dc.language.isoeng
dc.relation.ispartofFrontiers in Cell and Developmental Biology
dc.sourceScopus
dc.subjectCLDN-1
dc.subjectepididymis
dc.subjecthormone receptors
dc.subjectmaternal protein restriction
dc.subjectpostnatal development
dc.subjectsrc
dc.titleMaternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manneren
dc.typeArtigo

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