Formulation, stability study and preclinical evaluation of a vaginal cream containing curcumin in a rat model of vulvovaginal candidiasis

dc.contributor.authorFernandes, Ligia de Souza [UNESP]
dc.contributor.authorAmorim, Yuri Martins
dc.contributor.authorSilva, Elton Liberio da
dc.contributor.authorSilva, Samuel Calixto
dc.contributor.authorAparecida Santos, Alecia Junia
dc.contributor.authorPeixoto, Franciele Natalia
dc.contributor.authorNeves Pires, Luara Moniele
dc.contributor.authorSakamoto, Raquel Yumi
dc.contributor.authorHorta Pinto, Flavia do Carmo
dc.contributor.authorCosta Scarpa, Maria Virginia [UNESP]
dc.contributor.authorFreitas Araujo, Marcelo Gonzaga de
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Fed Sao Joao del Rei
dc.date.accessioned2018-11-26T17:55:33Z
dc.date.available2018-11-26T17:55:33Z
dc.date.issued2018-10-01
dc.description.abstractOwing to the growing resistance among isolates of Candida species to usual antifungal agents and the well-known therapeutic potential of curcumin, the purpose of this study was to develop and validate a vaginal formulation containing this substance and to evaluating its effectiveness in the treatment of experimental vulvovaginal candidiasis. Curcumin was incorporated in a vaginal cream in three concentrations (0.01%, 0.1% and 1.0%). The different concentrations of the cream and its controls were intravaginally administered in an immunosuppressed rat model to evaluate the efficacy in the treatment of experimental vulvovaginal candidiasis. Samples of the cream were also subjected to centrifugation and physical stability tests and an analytical method for quantification of curcumin was validated based on HPLC. The formulation was stable and the HPLC method could be considered suitable for the quantitative determination of curcumin in the cream. After 6days of preclinical study, the number of infected animals was 1/6 in all groups treated with curcumin vaginal cream and the fungal burden showed a progressive reduction. Reduction in the inflammatory infiltrate was observed in the group treated with 1.0% cream. Vaginal cream containing curcumin could be considered a promising effective antifungal medicine in the treatment of vulvovaginal candidiasis.en
dc.description.affiliationSao Paulo State Univ, UNESP, Dept Drugs & Pharmaceut, Fac Pharmaceut Sci, Araraquara, SP, Brazil
dc.description.affiliationUniv Fed Sao Joao del Rei, Lab Pharmacol, Campus Ctr Oeste Dona Lindu, Divinopolis, MG, Brazil
dc.description.affiliationUniv Fed Sao Joao del Rei, Nat Sci Dept, Campus Dom Bosco, Sao Joao Del Rei, MG, Brazil
dc.description.affiliationUnespSao Paulo State Univ, UNESP, Dept Drugs & Pharmaceut, Fac Pharmaceut Sci, Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipUFSJ
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent723-730
dc.identifierhttp://dx.doi.org/10.1111/myc.12762
dc.identifier.citationMycoses. Hoboken: Wiley, v. 61, n. 10, p. 723-730, 2018.
dc.identifier.doi10.1111/myc.12762
dc.identifier.issn0933-7407
dc.identifier.urihttp://hdl.handle.net/11449/164673
dc.identifier.wosWOS:000445333300003
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofMycoses
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectanalytical method validation
dc.subjectantifungal
dc.subjectCandida albicans
dc.subjectCurcumin
dc.subjectstability study
dc.subjectvaginal cream
dc.subjectvulvovaginal candidiasis
dc.titleFormulation, stability study and preclinical evaluation of a vaginal cream containing curcumin in a rat model of vulvovaginal candidiasisen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.orcid0000-0002-7072-0922[1]

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