Serratia liquefaciens FG3 isolated from a metallophyte plant sheds light on the evolution and mechanisms of adaptive traits in extreme environments

dc.contributor.authorCaneschi, Washington Luiz
dc.contributor.authorSanchez, Angélica Bianchini
dc.contributor.authorFelestrino, Érica Barbosa
dc.contributor.authorLemes, Camila Gracyelle de Carvalho
dc.contributor.authorCordeiro, Isabella Ferreira
dc.contributor.authorFonseca, Natasha Peixoto
dc.contributor.authorVilla, Morghana Marina
dc.contributor.authorVieira, Izadora Tabuso
dc.contributor.authorMoraes, Lauro Ângelo Gonçalves
dc.contributor.authorAssis, Renata de Almeida Barbosa
dc.contributor.authordo Carmo, Flávio Fonseca
dc.contributor.authorKamino, Luciana Hiromi Yoshino
dc.contributor.authorSilva, Robson Soares
dc.contributor.authorFerro, Jesus Aparecido [UNESP]
dc.contributor.authorFerro, Maria Inês Tiraboschi [UNESP]
dc.contributor.authorFerreira, Rafael Marini [UNESP]
dc.contributor.authorSantos, Vera Lúcia
dc.contributor.authorSilva, Ubiana de Cássia Mourão
dc.contributor.authorAlmeida, Nalvo Franco
dc.contributor.authorVarani, Alessandro de Mello [UNESP]
dc.contributor.authorGarcia, Camila Carrião Machado
dc.contributor.authorSetubal, João Carlos
dc.contributor.authorMoreira, Leandro Marcio
dc.contributor.institutionUniversidade Federal de Ouro Preto (UFOP)
dc.contributor.institutionInstituto Prístino
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionVirginia Tech
dc.description.abstractSerratia liquefaciens strain FG3 (SlFG3), isolated from the flower of Stachytarpheta glabra in the Brazilian ferruginous fields, has distinctive genomic, adaptive, and biotechnological potential. Herein, using a combination of genomics and molecular approaches, we unlocked the evolution of the adaptive traits acquired by S1FG3, which exhibits the second largest chromosome containing the largest conjugative plasmids described for Serratia. Comparative analysis revealed the presence of 18 genomic islands and 311 unique protein families involved in distinct adaptive features. S1FG3 has a diversified repertoire of genes associated with Nonribosomal peptides (NRPs/PKS), a complete and functional cluster related to cellulose synthesis, and an extensive and functional repertoire of oxidative metabolism genes. In addition, S1FG3 possesses a complete pathway related to protocatecuate and chloroaromatic degradation, and a complete repertoire of genes related to DNA repair and protection that includes mechanisms related to UV light tolerance, redox process resistance, and a laterally acquired capacity to protect DNA using phosphorothioation. These findings summarize that SlFG3 is well-adapted to different biotic and abiotic stress situations imposed by extreme conditions associated with ferruginous fields, unlocking the impact of the lateral gene transfer to adjust the genome for extreme environments, and providing insight into the evolution of prokaryotes.en
dc.description.affiliationNúcleo de Pesquisas em Ciências Biológicas (NUPEB) Universidade Federal de Ouro Preto (UFOP)
dc.description.affiliationInstituto Prístino
dc.description.affiliationFaculdade de Computação (FACOM) Universidade Federal de Mato Grosso do Sul
dc.description.affiliationFaculdade de Ciências Agrárias e Veterinárias de Jaboticabal UNESP – Universidade Estadual Paulista Departamento de Tecnologia
dc.description.affiliationDepartamento de Microbiologia Universidade Federal de Minas Gerais (UFMG)
dc.description.affiliationDepartamento de Ciências Biológicas (DECBI) Instituto de Ciências Exatas e Biológicas (ICEB) Universidade Federal de Ouro Preto (UFOP)
dc.description.affiliationDepartamento de Bioquímica (DB) Instituto de Química (IQ) Universidade de São Paulo (USP)
dc.description.affiliationBiocomplexity Institute Virginia Tech
dc.description.affiliationUnespFaculdade de Ciências Agrárias e Veterinárias de Jaboticabal UNESP – Universidade Estadual Paulista Departamento de Tecnologia
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPEMIG: APQ-02387-14/APQ-02357-17
dc.description.sponsorshipIdCAPES: CFP 51/2013
dc.description.sponsorshipIdCAPES: process 3385/2013
dc.identifier.citationScientific Reports, v. 9, n. 1, 2019.
dc.relation.ispartofScientific Reports
dc.titleSerratia liquefaciens FG3 isolated from a metallophyte plant sheds light on the evolution and mechanisms of adaptive traits in extreme environmentsen
unesp.departmentTecnologia - FCAVpt