Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity

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dc.contributor.authorTrigo-Gutierrez, Jeffersson Krishan [UNESP]
dc.contributor.authorCalori, Italo Rodrigo
dc.contributor.authorde Oliveira Bárbara, Geovana [UNESP]
dc.contributor.authorPavarina, Ana Claudia [UNESP]
dc.contributor.authorGonçalves, Renato Sonchini
dc.contributor.authorCaetano, Wilker
dc.contributor.authorTedesco, Antonio Claudio
dc.contributor.authorMima, Ewerton Garcia de Oliveira [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionState University of Maringá
dc.date.accessioned2023-07-29T16:13:08Z
dc.date.available2023-07-29T16:13:08Z
dc.date.issued2023-01-01
dc.description.abstractNanocarriers have been successfully used to solubilize, deliver, and increase the bioavailability of curcumin (CUR), but slow CUR release rates hinder its use as a topical photosensitizer in antimicrobial photodynamic therapy. A photo-responsive polymer (PRP) was designed for the light-triggered release of CUR with an effective light activation-dependent antimicrobial response. The characterization of the PRP was compared with non-responsive micelles comprising Pluronics™ P123 and F127. According to the findings, the PRP formed photo-responsive micelles in the nanometric scale (< 100 nm) with a lower critical micelle concentration (3.74 × 10−4 M−1, 5.8 × 10−4 M−1, and 7.2 × 10−6 M−1 for PRP, F127, P123, respectively, at 25°C) and higher entrapment efficiency of CUR (88.7, 77.2, and 72.3% for PRP, F127, and P123 micelles, respectively) than the pluronics evaluated. The PRP provided enhanced protection of CUR compared to P123 micelles, as demonstrated in fluorescence quenching studies. The light-triggered release of CUR from PRP occurred with UV light irradiation (at 355 nm and 25 mW cm−2) and a cumulative release of 88.34% of CUR within 1 h compared to 80% from pluronics after 36 h. In vitro studies showed that CUR-loaded PRP was non-toxic to mammal cell, showed inactivation of the pathogenic microorganisms Candida albicans, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus, and decreased biofilm biomass when associated with blue light (455 nm, 33.84 J/cm2). The findings show that the CUR-loaded PRP micelle is a viable option for antimicrobial activity.en
dc.description.affiliationLaboratory of Applied Microbiology Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Chemistry Center of Nanotechnology and Tissue Engineering Photobiology and Photomedicine Research Group Faculty of Philosophy Sciences and Letters of Ribeirão Preto University of São Paulo, São Paulo
dc.description.affiliationDepartment of Chemistry Research Nucleus of Photodynamic Therapy State University of Maringá, Paraná
dc.description.affiliationUnespLaboratory of Applied Microbiology Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2023.1132781
dc.identifier.citationFrontiers in Microbiology, v. 14.
dc.identifier.doi10.3389/fmicb.2023.1132781
dc.identifier.issn1664-302X
dc.identifier.scopus2-s2.0-85158009632
dc.identifier.urihttp://hdl.handle.net/11449/249931
dc.language.isoeng
dc.relation.ispartofFrontiers in Microbiology
dc.sourceScopus
dc.subjectbacteria
dc.subjectfungi
dc.subjectlight
dc.subjectmicelles
dc.subjectPhotochemotherapy
dc.titlePhoto-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activityen
dc.typeArtigo

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