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Sex determination of murine and bovine embryos using cytotoxicity and immunofluorescence assays

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dc.contributor.authorHossepian de Lima, V. F M
dc.contributor.authorMoreira-Filho, C. A.
dc.contributor.authorDe Bem, A. R.
dc.contributor.authorJorge, W. [UNESP]
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:17:53Z
dc.date.available2014-05-27T11:17:53Z
dc.date.issued1993-06-01
dc.description.abstractMurine and bovine embryos were sexed by using polyclonal and monoclonal anti-H-Y antibodies in cytotoxic and immunofluorescence assays. The specificity of 3 different sources of antibodies are compared. Sexing was accomplished by complement-mediated cytotoxicity or by H-Y antigen detection using a anti-mouse Ig fluorescein conjugate. Cytogenetic ascertainment for correct sex identification yielded a score of about 85% in both species, while the immunofluorescence assay yielded a score of 83%. © 1993.en
dc.description.affiliationEmbrapa-Cenargen, Caixa Postal 02372, CEP 70770 Brasília, DF
dc.description.affiliationDepartamento de Imunologia ICB USP São Paulo, SP
dc.description.affiliationDepartamento de Genética UNESP Botucatu, SP
dc.description.affiliationUnespDepartamento de Genética UNESP Botucatu, SP
dc.format.extent1343-1352
dc.identifierhttp://dx.doi.org/10.1016/0093-691X(93)90236-X
dc.identifier.citationTheriogenology, v. 39, n. 6, p. 1343-1352, 1993.
dc.identifier.doi10.1016/0093-691X(93)90236-X
dc.identifier.issn0093-691X
dc.identifier.scopus2-s2.0-38249001637
dc.identifier.urihttp://hdl.handle.net/11449/64377
dc.identifier.wosWOS:A1993LF25800013
dc.language.isoeng
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectanti-H-Y antibodies
dc.subjectembryo
dc.subjectimmunological assays
dc.subjectsexing
dc.titleSex determination of murine and bovine embryos using cytotoxicity and immunofluorescence assaysen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.departmentGenética - IBBpt

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Botucatu - IBB - Instituto de Biociências