Melanin-concentrating hormone regulates the hypercapnic chemoreflex by acting in the lateral hypothalamic area

Dias, Mirela B. [UNESP]; Gargaglioni, Luciane H [UNESP]; Patrone, Luis G.A. [UNESP]; Rodrigues, Laísa TC

Melanin-concentrating hormone regulates the hypercapnic chemoreflex by acting in the lateral hypothalamic area

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dias_mb_dadodepesquisa_bot_mch3.pdf (622.96 KB)

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dias_mb_dadodepesquisa_bot_snap1.pdf (632.11 KB)

Data

2022

Autores

Dias, Mirela B.

Gargaglioni, Luciane H

Patrone, Luis G.A.

Rodrigues, Laísa TC

Tipo

Dado de pesquisa

Direito de acesso

Acesso aberto

Descrição

Melanin-concentrating hormone (MCH) is a hypothalamic neuropeptide involved in a broad range of homeostatic functions including regulation of the hypercapnic chemoreflex. We evaluated whether MCH modulates the hypercapnic ventilatory response by acting in the lateral hypothalamic area (LHA) and/or in the locus coeruleus (LC). Here, we measured pulmonary ventilation ( E), body temperature, EEG and EMG of unanesthetized male Wistar rats before and after microinjection of MCH [0.4 mM] into the LHA and LC, or the MCH1-R antagonist (SNAP-94847 [63 mM]), into the LHA, in room air and in 7% CO2 during wakefulness and sleep, in the dark and light periods. MCH intra-LHA caused a decreased CO2 ventilatory response during wakefulness and sleep, while SNAP-94847 intra-LHA increased this response, during wakefulness in the light period. In the LC, MCH or the MCH1-R antagonist caused no change in the hypercapnic ventilatory response. Our results suggest that MCH, in the LHA, exerts an inhibitory modulation of the hypercapnic ventilatory response during the light-inactive period in rats.