Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Rats

dc.contributor.authorBarbosa, Rafaela Moreira [UNESP]
dc.contributor.authorSperetta, Guilherme F. [UNESP]
dc.contributor.authorMartins Dias, Daniel Penteado
dc.contributor.authorRuchaya, Prashant Jay [UNESP]
dc.contributor.authorLi, Hongwei
dc.contributor.authorMenani, Jose Vanderlei [UNESP]
dc.contributor.authorSumners, Colin
dc.contributor.authorColombari, Eduardo [UNESP]
dc.contributor.authorColombari, Debora S. A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionSouthern Med Univ
dc.contributor.institutionUniv Florida
dc.date.accessioned2018-11-26T17:33:01Z
dc.date.available2018-11-26T17:33:01Z
dc.date.issued2017-04-01
dc.description.abstractBACKGROUND Macrophage migration inhibitory factor (MIF) is an intracellular inhibitory regulator of the actions of angiotensin II in the central nervous system. Renovascular hypertensive 2-kidney, 1-clip (2K1C) rats have an increased activity of the renin-angiotensin system and a decrease in baroreflex function compared to normotensive (NT) rats. In the present study, we tested the effects of MIF overexpression within the nucleus of the solitary tract (NTS), a key brainstem region for cardiovascular regulation, on the development of hypertension, on baroreflex function, and on water and food intake in 2K1C rats. METHODS Holtzman NT rats received a silver clip around the left renal artery to induce 2K1C hypertension. Three weeks later, rats were microinjected in the NTS with AAV2-CBA-MIF, to increase the expression of MIF, or with the control vector AAV2-CBA-enhanced green fluorescent protein. Mean arterial pressure (MAP) and heart rate were recorded by telemetry. Baroreflex function was tested, and water and food intake were also measured. RESULTS Increasing MIF expression in the NTS of 2K1C rats attenuated the development of hypertension, reversed the impairment of baroreflex function, and reduced the increase in water intake. In contrast to 2K1C rats, similar increases in MIF expression in the NTS of NT rats produced no changes in baseline MAP, baroreflex function, or water intake. CONCLUSIONS These results indicate that an increased expression of MIF within the NTS attenuates the development of hypertension and restores the baroreflex function in 2K1C rats.en
dc.description.affiliationSao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Ribeirao Preto Med Sch, Dept Physiol, Ribeirao Preto, SP, Brazil
dc.description.affiliationSouthern Med Univ, Sch Biotechnol, Guangzhou, Guangdong, Peoples R China
dc.description.affiliationUniv Florida, Coll Med, Dept Physiol & Funct Genom, Gainesville, FL USA
dc.description.affiliationUnespSao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipNIH
dc.description.sponsorshipIdCNPq: 473108/2011-9
dc.description.sponsorshipIdCNPq: 304918/2011-3
dc.description.sponsorshipIdFAPESP: 2011/50770-1
dc.description.sponsorshipIdFAPESP: 2015/23467-7
dc.description.sponsorshipIdNIH: HL-076803
dc.format.extent435-443
dc.identifierhttp://dx.doi.org/10.1093/ajh/hpx001
dc.identifier.citationAmerican Journal Of Hypertension. Oxford: Oxford Univ Press, v. 30, n. 4, p. 435-443, 2017.
dc.identifier.doi10.1093/ajh/hpx001
dc.identifier.fileWOS000402054800017.pdf
dc.identifier.issn0895-7061
dc.identifier.urihttp://hdl.handle.net/11449/162833
dc.identifier.wosWOS:000402054800017
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofAmerican Journal Of Hypertension
dc.relation.ispartofsjr1,322
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectangiotensin II
dc.subjectAT1 receptor
dc.subjectbaroreflex
dc.subjectblood pressure
dc.subjectbrainstem
dc.subjecthypertension
dc.subject2K1C
dc.titleIncreased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Ratsen
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dcterms.rightsHolderOxford Univ Press
unesp.author.lattes4544450092427426[8]
unesp.author.orcid0000-0002-1395-4036[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt
unesp.departmentFisiologia e Patologia - FOARpt

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
WOS000402054800017.pdf
Size:
1.82 MB
Format:
Adobe Portable Document Format
Description: