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Master regulators of epithelial-mesenchymal transition and wnt signaling pathways in juvenile nasopharyngeal angiofibromas

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dc.contributor.authorCalanca, Naiade [UNESP]
dc.contributor.authorBinato, Sara Martoreli Silveira
dc.contributor.authorSilva, Sabrina Daniela da
dc.contributor.authorBrentani, Helena Paula
dc.contributor.authorSennes, Luiz Ubirajara
dc.contributor.authorPinto, Clóvis Antonio Lopes
dc.contributor.authorDomingues, Maria Aparecida Custódio [UNESP]
dc.contributor.authorFonseca-Alves, Carlos Eduardo [UNESP]
dc.contributor.authorRainho, Claudia Aparecida [UNESP]
dc.contributor.authorRogatto, Silvia Regina
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionA.C. Camargo Cancer Center
dc.contributor.institutionSir Mortimer B. Davis-Jewish General Hospital
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionPaulista University—UNIP
dc.contributor.institutionUniversity Hospital of Southern Denmark
dc.contributor.institutionUniversity of Southern Denmark
dc.date.accessioned2022-04-29T08:45:57Z
dc.date.available2022-04-29T08:45:57Z
dc.date.issued2021-09-01
dc.description.abstractJuvenile nasopharyngeal angiofibroma (JNA) is a rare fibrovascular benign tumor showing an invasive growth pattern and affecting mainly male adolescents. We investigated the role of epithelial–mesenchymal transition (EMT) and WNT signaling pathways in JNA. Gene expression profiles using nine JNA paired with four inferior nasal turbinate samples were interrogated using a customized 2.3K microarray platform containing genes mainly involved in EMT and WNT/PI3K pathways. The expression of selected genes (BCL2, CAV1, CD74, COL4A2, FZD7, ING1, LAMB1, and RAC2) and proteins (BCL2, CAV1, CD74, FZD7, RAF1, WNT5A, and WNT5B) was investigated by RT-qPCR (28 cases) and immunohistochemistry (40 cases), respectively. Among 104 differentially expressed genes, we found a significantly increased expression of COL4A2 and LAMB1 and a decreased expression of BCL2 and RAC2 by RT-qPCR. The immunohistochemistry analysis revealed a low expression of BCL2 and a negative to moderate expression of FZD7 in most samples, while increased CAV1 and RAF1 expression were detected. Moderate to strong CD74 protein expression was observed in endothelial and inflammatory cells. A significant number of JNAs (78%) presented reduced WNT5A and increased WNT5B expression. Overall, the transcript and protein profile indicated the involvement of EMT and WNT pathways in JNA. These candidates are promising druggable targets for treating JNA.en
dc.description.affiliationDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationInternational Research Center CIPE A.C. Camargo Cancer Center
dc.description.affiliationDepartment of Otolaryngology—Head and Neck Surgery McGill University Sir Mortimer B. Davis-Jewish General Hospital
dc.description.affiliationDepartment of Psychiatry LIM23 (FMUSP) University of Sao Paulo (USP)
dc.description.affiliationDepartment of Otorhinolaryngology FMUSP University of São Paulo (USP)
dc.description.affiliationDepartment of Pathology A.C. Camargo Cancer Center
dc.description.affiliationDepartment of Pathology Faculty of Medicine São Paulo State University (UNESP)
dc.description.affiliationInstitute of Health Sciences Paulista University—UNIP
dc.description.affiliationDepartment of Veterinary Surgery and Anesthesiology Sao Paulo State University (UNESP)
dc.description.affiliationDepartment of Clinical Genetics University Hospital of Southern Denmark
dc.description.affiliationInstitute of Regional Health Research University of Southern Denmark
dc.description.affiliationUnespDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Pathology Faculty of Medicine São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Veterinary Surgery and Anesthesiology Sao Paulo State University (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCAPES: 88887.310463/2018-00
dc.description.sponsorshipIdCAPES: 88887.374335/2019-00
dc.identifierhttp://dx.doi.org/10.3390/biomedicines9091258
dc.identifier.citationBiomedicines, v. 9, n. 9, 2021.
dc.identifier.doi10.3390/biomedicines9091258
dc.identifier.issn2227-9059
dc.identifier.scopus2-s2.0-85115755760
dc.identifier.urihttp://hdl.handle.net/11449/231519
dc.language.isoeng
dc.relation.ispartofBiomedicines
dc.sourceScopus
dc.subjectEpithelial–mesenchymal transition
dc.subjectGene expression profile
dc.subjectJuvenile nasopharyngeal angiofibroma
dc.subjectTumor microenvironment
dc.subjectTumor signaling pathways
dc.subjectWNT pathway
dc.titleMaster regulators of epithelial-mesenchymal transition and wnt signaling pathways in juvenile nasopharyngeal angiofibromasen
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.departmentNeurologia, Psicologia e Psiquiatria - FMBpt

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Botucatu - FMB - Faculdade de Medicina