Genomic and Genotypic Characterization of Cylindrospermopsis raciborskii: Toward an Intraspecific Phylogenetic Evaluation by Comparative Genomics

Página do item simplificado
dc.contributor.authorAbreu, Vinicius A. C.
dc.contributor.authorPopin, Rafael V.
dc.contributor.authorAlvarenga, Danillo O. [UNESP]
dc.contributor.authorSchaker, Patricia D. C.
dc.contributor.authorHoff-Risseti, Caroline
dc.contributor.authorVarani, Alessandro M. [UNESP]
dc.contributor.authorFiore, Marli F.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T15:47:33Z
dc.date.available2018-11-26T15:47:33Z
dc.date.issued2018-02-26
dc.description.abstractCylindrospermopsis raciborskii is a freshwater cyanobacterial species with increasing bloom reports worldwide that are likely due to factors related to climate change. In addition to the deleterious effects of blooms on aquatic ecosystems, the majority of ecotypes can synthesize toxic secondary metabolites causing public health issues. To overcome the harmful effects of C. raciborskii blooms, it is important to advance knowledge of diversity, genetic variation, and evolutionary processes within populations. An efficient approach to exploring this diversity and understanding the evolution of C. raciborskii is to use comparative genomics. Here, we report two new draft genomes of C. raciborskii (strains CENA302 and CENA303) from Brazilian isolates of different origins and explore their molecular diversity, phylogeny, and evolutionary diversification by comparing their genomes with sequences from other strains available in public databases. The results obtained by comparing seven C. raciborskii and the Raphidiopsis brookii D9 genomes revealed a set of conserved core genes and a variable set of accessory genes, such as those involved in the biosynthesis of natural products, heterocyte glycolipid formation, and nitrogen fixation. Gene cluster arrangements related to the biosynthesis of the antifungal cyclic glycosylated lipopeptide hassallidin were identified in four C. raciborskii genomes, including the non-nitrogen fixing strain CENA303. Shifts in gene clusters involved in toxin production according to geographic origins were observed, as well as a lack of nitrogen fixation (nif) and heterocyte glycolipid (hgl) gene clusters in some strains. Single gene phylogeny (16S rRNA sequences) was congruent with phylogeny based on 31 concatenated housekeeping protein sequences, and both analyses have shown, with high support values, that the species C. raciborskii is monophyletic. This comparative genomics study allowed a species-wide view of the biological diversity of C. raciborskii and in some cases linked genome differences to phenotype.en
dc.description.affiliationUniv Sao Paulo, Ctr Nucl Energy Agr, Piracicaba, Brazil
dc.description.affiliationSao Paulo State Univ, Sch Agr & Vet Sci, Jaboticabal, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Sch Agr & Vet Sci, Jaboticabal, Brazil
dc.description.sponsorshipNational Natural Science Foundation of China
dc.description.sponsorshipNatural Science Foundation of Hubei Province
dc.description.sponsorshipIdNational Natural Science Foundation of China: 31271406
dc.description.sponsorshipIdNational Natural Science Foundation of China: 31571351
dc.description.sponsorshipIdNatural Science Foundation of Hubei Province: 2015CFA044
dc.format.extent23
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2018.00306
dc.identifier.citationFrontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 9, 23 p., 2018.
dc.identifier.doi10.3389/fmicb.2018.00306
dc.identifier.fileWOS000426053900001.pdf
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/11449/160121
dc.identifier.wosWOS:000426053900001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Microbiology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectcyanobacteria
dc.subjectgenome assembly
dc.subjectpan-genome
dc.subjectbioinformatics
dc.subjectnatural products
dc.subjectcyanotoxins
dc.subjectnitrogen fixation
dc.titleGenomic and Genotypic Characterization of Cylindrospermopsis raciborskii: Toward an Intraspecific Phylogenetic Evaluation by Comparative Genomicsen
dc.typeArtigo
dcterms.rightsHolderFrontiers Media Sa
unesp.author.lattes9429712259649346[6]
unesp.author.orcid0000-0002-8876-3269[6]

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000426053900001.pdf
Tamanho:
1.69 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar

Coleções

Artigos