Effects of cyclosporin, nifedipine and phenytoin on gingival myofibroblast transdifferentiation in monkeys

dc.contributor.authorKanno, Claudia Misue [UNESP]
dc.contributor.authorOliveira, Jose Americo de [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorPires Soubhia, Ana Maria [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-04T12:32:17Z
dc.date.available2019-10-04T12:32:17Z
dc.date.issued2019-01-01
dc.description.abstractObjective: Myofibroblasts have been associated with the development of several pathologic fibrotic conditions. This longitudinal study aims to assess the proliferative and antiapoptotic effects of cyclosporin, nifedipine and phenytoin on gingival connective tissue cells of nonhuman primate, as well as to analyze a possible role of myofibroblasts in gingival overgrowth. Materials and Methods: Gingival samples from the right superior canine area were obtained from 12 male monkeys (Sapajus spp) to comprise the control group. After one week, the animals were randomly assigned to three groups, which received daily oral doses of cyclosporin, nifedipine or phenytoin for 120 days. Gingival samples were collected from the left superior canine area of two animals of each group at 52 and 120 days. Histological sections were stained with hematoxylin and eosin, and immunoreacted against alpha-SMA, Ki67 and bcl-2. Results: alpha-SMA immunoreaction was negative in the control and experimental groups. Similarly, no difference between groups concerning immunostaining against Ki-67 and bcl-2 was observed in connective tissue cells. Conclusion: Based on this methodology, it may be concluded that gingival overgrowths induced by cyclosporin, nifedipine and phenytoin are not associated with neither myofibroblast transdifferentiation, proliferation nor apoptosis of gingival connective cells in monkeys.en
dc.description.affiliationUniv Estadual Paulista, Fac Odontol, Dept Emergencia, Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Odontol, Dept Ciencias Basicas, Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Odontol, Dept Patol, Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Odontol, Propedeut Clin, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Odontol, Dept Emergencia, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Odontol, Dept Ciencias Basicas, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Odontol, Dept Patol, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Odontol, Propedeut Clin, Aracatuba, SP, Brazil
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1590/1678-7757-2018-0135
dc.identifier.citationJournal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 27, 8 p., 2019.
dc.identifier.doi10.1590/1678-7757-2018-0135
dc.identifier.fileS1678-77572019000100404.pdf
dc.identifier.issn1678-7757
dc.identifier.lattes4408095517346846
dc.identifier.orcid0000-0003-4859-0583
dc.identifier.scieloS1678-77572019000100404
dc.identifier.urihttp://hdl.handle.net/11449/185056
dc.identifier.wosWOS:000450078500001
dc.language.isoeng
dc.publisherUniv Sao Paulo Fac Odontologia Bauru
dc.relation.ispartofJournal Of Applied Oral Science
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectGingival overgrowth
dc.subjectCyclosporine
dc.subjectPhenytoin
dc.subjectNifedipine
dc.subjectMyofibroblast
dc.titleEffects of cyclosporin, nifedipine and phenytoin on gingival myofibroblast transdifferentiation in monkeysen
dc.typeArtigo
dcterms.rightsHolderUniv Sao Paulo Fac Odontologia Bauru
unesp.author.lattes4408095517346846[3]
unesp.author.orcid0000-0003-4859-0583[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt
unesp.departmentCiências Básicas - FOApt
unesp.departmentPatologia e Propedêutica Clínica - FOApt

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