Injectable MMP-Responsive Nanotube-Modified Gelatin Hydrogel for Dental Infection Ablation

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dc.contributor.authorRibeiro, Juliana S.
dc.contributor.authorBordini, Ester A. F. [UNESP]
dc.contributor.authorFerreira, Jessica A.
dc.contributor.authorMei, Ling
dc.contributor.authorDubey, Nileshkumar
dc.contributor.authorFenno, J. Christopher
dc.contributor.authorPiva, Evandro
dc.contributor.authorLund, Rafael G.
dc.contributor.authorSchwendeman, Anna
dc.contributor.authorBottino, Marco C.
dc.contributor.institutionUniv Michigan
dc.contributor.institutionUniv Fed Pelotas
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-10T19:56:09Z
dc.date.available2020-12-10T19:56:09Z
dc.date.issued2020-04-08
dc.description.abstractA photocrosslinkable gelatin methacryloyl (GelMA) hydrogel has been widely examined in regenerative engineering because of its good cell-tissue affinity and degradability in the presence of matrix metalloproteinases. A halloysite aluminosilicate nanotube (HNT) is a known reservoir for the loading and sustained delivery of therapeutics. Here, we formulate injectable chlorhexidine (CHX)-loaded nanotube-modified GelMA hydrogel that is cytocompatible and biodegradable and provides sustained release of CHX for infection ablation while displaying good biocompatibility. The effects of HNTs and CHX on hydrogel degradability and mechanical properties, as well as on the kinetics of CHX release, and on the antimicrobial efficacy against oral pathogens were systematically assessed. Cytocompatibility in stem cells from human exfoliated deciduous teeth and inflammatory response in vivo using a subcutaneous rat model were determined. Our hydrogel system, that is, (CHX)-loaded nanotube-modified GelMA showed minimum localized inflammatory responses, supporting its ability for drug delivery applications. Moreover, we showed that the incorporation of CHX-loaded nanotubes reduces the mechanical properties, increases the swelling ratio, and diminishes the degradation rate of the hydrogels. Importantly, the presence of CHX-loaded nanotubes inhibits bacterial growth with minimal cell toxicity. Our findings provide a new strategy to modify GelMA hydrogel with chlorhexidine-loaded nanotubes for clinical use as an injectable drug delivery strategy for dental infection ablation.en
dc.description.affiliationUniv Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA
dc.description.affiliationUniv Fed Pelotas, Sch Dent, Dept Restorat Dent, BR-96010610 Pelotas, RS, Brazil
dc.description.affiliationSao Paulo State Univ, Sch Dent, Dept Dent Mat & Prosthodont, BR-01049010 Araraquara, SP, Brazil
dc.description.affiliationUniv Michigan, Coll Pharm, Dept Pharmaceut Sci, Ann Arbor, MI 48109 USA
dc.description.affiliationUniv Michigan, Biointerfaces Inst, Ann Arbor, MI 48109 USA
dc.description.affiliationUniv Michigan, Sch Dent, Dept Biol & Mat Sci & Prosthodont, Ann Arbor, MI 48109 USA
dc.description.affiliationUnespSao Paulo State Univ, Sch Dent, Dept Dent Mat & Prosthodont, BR-01049010 Araraquara, SP, Brazil
dc.description.sponsorshipNational Institutes of Health (NIH, National Institute of Dental and Craniofacial Research/NIDCR)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdNational Institutes of Health (NIH, National Institute of Dental and Craniofacial Research/NIDCR): K08DE023552
dc.description.sponsorshipIdNational Institutes of Health (NIH, National Institute of Dental and Craniofacial Research/NIDCR): R01DE026578
dc.format.extent16006-16017
dc.identifierhttp://dx.doi.org/10.1021/acsami.9b22964
dc.identifier.citationAcs Applied Materials & Interfaces. Washington: Amer Chemical Soc, v. 12, n. 14, p. 16006-16017, 2020.
dc.identifier.doi10.1021/acsami.9b22964
dc.identifier.issn1944-8244
dc.identifier.urihttp://hdl.handle.net/11449/196785
dc.identifier.wosWOS:000526583500004
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofAcs Applied Materials & Interfaces
dc.sourceWeb of Science
dc.subjectdrug delivery
dc.subjecthydrogel
dc.subjectinfection
dc.subjectendodontics
dc.subjectdentistry
dc.subjectmatrix metalloproteinases
dc.titleInjectable MMP-Responsive Nanotube-Modified Gelatin Hydrogel for Dental Infection Ablationen
dc.typeArtigo
dcterms.rightsHolderAmer Chemical Soc
unesp.author.orcid0000-0002-6664-1375[5]
unesp.author.orcid0000-0002-8023-8080[9]

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