Injectable MMP-Responsive Nanotube-Modified Gelatin Hydrogel for Dental Infection Ablation
dc.contributor.author | Ribeiro, Juliana S. | |
dc.contributor.author | Bordini, Ester A. F. [UNESP] | |
dc.contributor.author | Ferreira, Jessica A. | |
dc.contributor.author | Mei, Ling | |
dc.contributor.author | Dubey, Nileshkumar | |
dc.contributor.author | Fenno, J. Christopher | |
dc.contributor.author | Piva, Evandro | |
dc.contributor.author | Lund, Rafael G. | |
dc.contributor.author | Schwendeman, Anna | |
dc.contributor.author | Bottino, Marco C. | |
dc.contributor.institution | Univ Michigan | |
dc.contributor.institution | Univ Fed Pelotas | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2020-12-10T19:56:09Z | |
dc.date.available | 2020-12-10T19:56:09Z | |
dc.date.issued | 2020-04-08 | |
dc.description.abstract | A photocrosslinkable gelatin methacryloyl (GelMA) hydrogel has been widely examined in regenerative engineering because of its good cell-tissue affinity and degradability in the presence of matrix metalloproteinases. A halloysite aluminosilicate nanotube (HNT) is a known reservoir for the loading and sustained delivery of therapeutics. Here, we formulate injectable chlorhexidine (CHX)-loaded nanotube-modified GelMA hydrogel that is cytocompatible and biodegradable and provides sustained release of CHX for infection ablation while displaying good biocompatibility. The effects of HNTs and CHX on hydrogel degradability and mechanical properties, as well as on the kinetics of CHX release, and on the antimicrobial efficacy against oral pathogens were systematically assessed. Cytocompatibility in stem cells from human exfoliated deciduous teeth and inflammatory response in vivo using a subcutaneous rat model were determined. Our hydrogel system, that is, (CHX)-loaded nanotube-modified GelMA showed minimum localized inflammatory responses, supporting its ability for drug delivery applications. Moreover, we showed that the incorporation of CHX-loaded nanotubes reduces the mechanical properties, increases the swelling ratio, and diminishes the degradation rate of the hydrogels. Importantly, the presence of CHX-loaded nanotubes inhibits bacterial growth with minimal cell toxicity. Our findings provide a new strategy to modify GelMA hydrogel with chlorhexidine-loaded nanotubes for clinical use as an injectable drug delivery strategy for dental infection ablation. | en |
dc.description.affiliation | Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA | |
dc.description.affiliation | Univ Fed Pelotas, Sch Dent, Dept Restorat Dent, BR-96010610 Pelotas, RS, Brazil | |
dc.description.affiliation | Sao Paulo State Univ, Sch Dent, Dept Dent Mat & Prosthodont, BR-01049010 Araraquara, SP, Brazil | |
dc.description.affiliation | Univ Michigan, Coll Pharm, Dept Pharmaceut Sci, Ann Arbor, MI 48109 USA | |
dc.description.affiliation | Univ Michigan, Biointerfaces Inst, Ann Arbor, MI 48109 USA | |
dc.description.affiliation | Univ Michigan, Sch Dent, Dept Biol & Mat Sci & Prosthodont, Ann Arbor, MI 48109 USA | |
dc.description.affiliationUnesp | Sao Paulo State Univ, Sch Dent, Dept Dent Mat & Prosthodont, BR-01049010 Araraquara, SP, Brazil | |
dc.description.sponsorship | National Institutes of Health (NIH, National Institute of Dental and Craniofacial Research/NIDCR) | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorshipId | National Institutes of Health (NIH, National Institute of Dental and Craniofacial Research/NIDCR): K08DE023552 | |
dc.description.sponsorshipId | National Institutes of Health (NIH, National Institute of Dental and Craniofacial Research/NIDCR): R01DE026578 | |
dc.format.extent | 16006-16017 | |
dc.identifier | http://dx.doi.org/10.1021/acsami.9b22964 | |
dc.identifier.citation | Acs Applied Materials & Interfaces. Washington: Amer Chemical Soc, v. 12, n. 14, p. 16006-16017, 2020. | |
dc.identifier.doi | 10.1021/acsami.9b22964 | |
dc.identifier.issn | 1944-8244 | |
dc.identifier.uri | http://hdl.handle.net/11449/196785 | |
dc.identifier.wos | WOS:000526583500004 | |
dc.language.iso | eng | |
dc.publisher | Amer Chemical Soc | |
dc.relation.ispartof | Acs Applied Materials & Interfaces | |
dc.source | Web of Science | |
dc.subject | drug delivery | |
dc.subject | hydrogel | |
dc.subject | infection | |
dc.subject | endodontics | |
dc.subject | dentistry | |
dc.subject | matrix metalloproteinases | |
dc.title | Injectable MMP-Responsive Nanotube-Modified Gelatin Hydrogel for Dental Infection Ablation | en |
dc.type | Artigo | |
dcterms.rightsHolder | Amer Chemical Soc | |
unesp.author.orcid | 0000-0002-6664-1375[5] | |
unesp.author.orcid | 0000-0002-8023-8080[9] |